Laser Imaging and Vibrational Spectroscopy Facility

Axioplan-2: Upright Fluorescent Microscope

Axioplan-2 microscopeAxioplan-2 computer, etc.

Model: Zeiss Axioplan 2
Location: 108B Partners III
Methodology and Rate: Microscopy (MIC) -

Current Instrument Status: NON-Operational – being repaired

Training

Operating Procedures

  • General Axioplan-2 operating procedures
  • Methodology specific operating procedures

Helpful Links

The Axioplan 2 is an upright stage fluorescent microscope, which is equipped with two digital cameras, Hamamatsu ORCA-ER and Zeiss Axiocam HRc. It is normally configured with the following objectives: 20X; 40X; 100X-oil; 50X-ref, and 100X-ref. The following standard filter sets DAPI, FITC, Rhodamine are available for use. This instrument is used most often for reflection, routine sample, and fluorescent measurements.

Applications

  • Reflection
  • Routine sample
  • Fluorescent measurements

Configurations

  • Standard Objectives
    • 20X visible light 0.50na 0.17 Plan-Neufluar
    • 40X visible light 0.85na Pol 0.17 Plan-Neufluar
    • 100X visible light 1.40na oil DIC, 0.17 Plan-Apochromat
    • 50X visible light 0.90na HD DIC Epiplan-Apochromat
    • 100X visible light 1.30na oil DIC 0.17 Plan-Neufluar

Optical Filters

  • DAPI
  • FITC
  • Rhodamine

Cameras

  • Hamamatsu ORCA-ER
  • Zeiss Axiocam HRc

Light Sources

  • White light
  • 75W Xenon arc lamp

Additional

  • Polarization
  • DIC