Axioplan-2: Upright Fluorescent Microscope
Model: Zeiss Axioplan 2
Location: 108B Partners III
Methodology and Rate: Microscopy (MIC) -
Current Instrument Status: NON-Operational – being repaired
Training
- Request Training for Axioplan-2
- Training Checklist for Axioplan-2
Operating Procedures
- General Axioplan-2 operating procedures
- Methodology specific operating procedures
Helpful Links
The Axioplan 2 is an upright stage fluorescent microscope, which is equipped with two digital cameras, Hamamatsu ORCA-ER and Zeiss Axiocam HRc. It is normally configured with the following objectives: 20X; 40X; 100X-oil; 50X-ref, and 100X-ref. The following standard filter sets DAPI, FITC, Rhodamine are available for use. This instrument is used most often for reflection, routine sample, and fluorescent measurements.
Applications
- Reflection
- Routine sample
- Fluorescent measurements
Configurations
- Standard Objectives
- 20X visible light 0.50na 0.17 Plan-Neufluar
- 40X visible light 0.85na Pol 0.17 Plan-Neufluar
- 100X visible light 1.40na oil DIC, 0.17 Plan-Apochromat
- 50X visible light 0.90na HD DIC Epiplan-Apochromat
- 100X visible light 1.30na oil DIC 0.17 Plan-Neufluar
Optical Filters
- DAPI
- FITC
- Rhodamine
Cameras
- Hamamatsu ORCA-ER
- Zeiss Axiocam HRc
Light Sources
- White light
- 75W Xenon arc lamp
Additional
- Polarization
- DIC
