Axiovert 200M: Inverted Confocal Microscope

Model: Zeiss Axiovert 200M
Location: 108C Partners III
Methodology and Rate: Microscopy (MIC) -

Current Instrument Status: Operational

Training

• Request Training for Axiovert 200M
• Training Checklist for Axiovert 200M

Operating Procedures

• General Axiovert 200M operating procedures
• Methodology specific operating procedures

Helpful Links

• General Information on Microscopy
• Optical Microscopy Primer
• A Microscope Tutorial

The Axiovert 200M is an inverted stage confocal microscope, which is equipped with three PMT’s and a digital camera, Zeiss Axiocam HRm and is fully automated. It is normally configured with the following objectives: 5X; 10X; 20X; 40X-H₂O; and 63X- H₂O. In addition to the laser scanning confocal capabilities the following standard filter sets DAPI, FITC, Rhodamine are available for use. This instrument is equipped with CO₂ incubator for live cell imaging. This instrument is used most often for confocal and fluorescent measurements, both with live cells and fixed samples.

Applications

• Live cell imaging
• Confocal fluorescent
• Fluorescent measurements

Configurations

• Standard Objectives
  • 5X visible light 0.15na 017 EC Plan-Neufluar
  • 10X visible light 0.30na 0.17 Plan-Neufluar
  • 20X visible light 0.75na 0.17 Plan-Apochromat
  • 40X visible light 1.20na W korr 0.14-0.18 C-Apochromat
  • 63X visible light 1.20na W korr 0.15-0.17 C-Apochromat

Optical Filters

• DAPI
• FITC
• Rhodamine

Cameras

• Zeiss Axiocam HRm
• 3-PMTs

Light Sources

• White light
• 75W Xenon arc lamp
• HeNe 543nm
• Argon Ion Laser 514, 488, 458nm
• Diode 405nm

Additional

• Polarization
• DIC