Mass spectrometry is a powerful analytical technique for the identification, characterization and quantification of minute quantities of analytes in pure form or in complex mixtures. The North Carolina State University Mass Spectrometry Facility (NCSU MSF), located on the ground floor of Dabney Hall, offers a variety of mass spectrometers providing analysis through an array of methods for sample introduction, ionization and mass measurement.
Our mass spectrometers include the following: (1) Thermo Fisher Scientific Exactive Plus MS; ESI with a mass range of 50 - 6,000 m/z; resolution: > 17,500 - 140,000 FWHM (200 m/z); mass accuracy: < 1 ppm RMS internally calibrated (2) Thermo Fisher Scientific LTQ Orbitrap XL; nanoflow or capillary ESI with a mass range of 50-2,000 m/z or 200-4,000 m/z; resolution: 7,500 - 100,000 FWHM (400 m/z); mass accuracy: < 2 ppm RMS internally calibrated via lock mass (3) Agilent Technologies 5975 GC/MS; EI, CI, NCI with a mass range 2 - 1,000 amu (4) Thermo Fisher Scientific TSQ Quantum Discovery MAX Triple Quadrupole MS; nanoflow or capillary ESI with a mass range of 10 - 3,000 m/z (5) AB Sciex 5800 MALDI-TOF/TOF MS; mass range of 0 - 200,000 m/z; resolution: > 33,000 FWHM (2,465 m/z) (6) Agilent Technologies GCD; EI with a mass range of 2 – 450 amu.
An array of services are provided to all University faculty, staff and students in the investigation of high molecular weight biological analytes such as peptides, proteins, oligonucleotides, complex lipids and carbohydrates, as well as synthetic polymers and small molecules. Additionally, the facility undertakes collaborative projects in the OMICS fields. The MSF is directed by a full-time mass spectrometrist - Dr. Taufika Islam Williams.