NCSU NMR TUTORIALS

NMR Tutorials

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High Resolution NMR
Solid-State NMR
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Using 500 MHz spectrometers

This 500 MHz Tutorial in print-friendly PDF format.

Bruker On-Line Manual

Basic ¹H Acquisition and Processing

I. Inserting the Sample

II. Spectrometer and Acquisition Parameters

III. Locking and Shimming

IV. Acquisition

V. Processing

VI. Plotting Spectra

Using 300 MHz spectrometers

This 300 MHz Tutorial in print-friendly PDF format.

BasicExperiment Set Up

I. Entering the sample into the magnet

A. Type "ej" to eject the old sample

II. Setting the shim and lock

III. Getting the proton spectra

A. Type "stdh" to get proton spectra

B. It will ask you how many acquistions you want, then type the number of acquistions needed in multiples of 4

C. When acquiring data has finished, type "save filename", where filename is equal to the name you want to give the file in the defined directory (/home/general/data/[PI]/[user])

E. Type "dis" to display FID

F. Type "See" to get the Fourier Transform. You will see the spectrum on the screen.

G. Type "srm" when you are done

IV. Getting the carbon spectra

A. Type "Stdc1" to get the carbon spectra

V. Analyzing the spectra

A. Hold the right mouse button on the display panel (area containing the spectra) and select "full," to get the entire spectra on the screen

1. select "adjust slope" with the left mouse button

d. Adjust the curvature

1. select "adjust curvature" with the left mouse button

e. Hit "select break points manual" with the left mouse button

quit setting break points

h. To select integral value

1. place cursor on peak

2. hold the right mouse button down, and then select "set integral value"

3. type the desired value

4. to print select "print"

5. to exit select "OK"

VI. Peak picking

A. Hold the "process" button with the right mouse button and select "peak picking"

C. Press the right mouse button to set

D. Press "find peaks" with the left mouse button to find all of the peaks that are above the threshold

VII. Printing

Commands for chge300 SUN for NMR

Command	Function
aph	autophase
acq	acquisition
apod_panel	brings up apodization panel
al	automatic lock
bacq	background mode -f -s (#scans) -d (# dummy scans) -p
bcorr	baseline correct
bcorr_panel	brings up baseline correction panel
bc;zerodata -c	zeros data and baseline corrects simultaneously
bigwin	increase size of keyboard win
c (clear)	clears screen
cd	change directory
cp	copy file
cb	change block size
da	gives data aquisition parameters: da -a, da \| \| pr -> prints da
deltav	set to one half sweep width for 1-1 Hard Pulse expt.
df	disk free
dg	sets decoupler
digpar	set aquisition parameters -a -b (block size)
dig read	read data to top of stack
dis	displays FID to screen
ej	eject sample (-f =keeps VT and spin rate on)
exp	gets experimenta -c, -d
export	file 1 [file 2] -b (for FELIX)
f	full screen
f1, f2	sets frequency(s) +/- x to increase/decrease
ft	Fouier transforms data
ez_panel	brings up easy data aquisition panel
get	gets a data file (-w = in writeable mode)
gn	gain
gamma	gammaH2 decoupling field strength
getshim	gets shim file
getcshim	gets compushim file
lf	gives linewidth of peak
ls	lists files in directory
mv	moves or renames file
na	number of aquisitions
peak_panel	brings up peak panel
peaks	finds peaks
pe	brings up phase panel
pd	pulse delay
plotparm	displays plot parameters
phase	phases data
process_panel	brings up process panel
presat	presaturates sample
printshim \| lpr	prints shim
pw	pulse width (90*)
rcp	remote copy
rg	receiver gate
rm	removes (deletes) files
rms	calculates root mean square
reverse	reverses spectra
run	begins aquisition
save	saves data as [filename]
see	Fouier transforms data and displays to screen
setinst	sets/removes instrument from data aquisition controling mode
sg	automatic gain adjustment
smooth	smoothes data
sn	gives siganl to noise ratio
spin	gives spin rate
spline	performs cubic spline on data (baseline correction)
splot	brings up panel to
srm	stack remove
ss	sets spin rate
sw	sweep width
tt	tells time experiment will take
za	gives aquisition parameters; za -a, za \| \| pr -> prints za
zerodata	zeros data (-c - between cursors)
zg	zero memory, go -> begins experiment

Shim Order for 2D Shimming

Nonspinning Shims:
X, ZX,
Y, ZY
^-Z²X, Z²Y
ZR², R²
X, X³
Y, Y³
^{ZXY, XY}

Misc. Command Routines:

To save an experiment to a filename while running:

To look at data while running (only initially):
set experiment parameters (ie. za, da, fl)
save [filename]
get -w [filename]
setinst
can change: gn, temp, na
na = x
run
digread -s (sees) digread -b (set to background)

To copy from chge300 to chsparc2:
rcp [filename] chsparc2:/alpha/omega/hardin

To copy from chsparc2 to chge300:
rcp [filename] chge300:/home/omega/data/user/

Gradient Spectroscopy

Pulsed field gradients (PFG) were introduced into NMR imaging more than 20 years ago. A number of technical problems had to be overcome before gradients could be successfully incorporated into high-resolution NMR studies.(1) The major technical problem comes from the fact that the application of PFG in static field B₀interferes with the receiver and the lock systems of the spectrometer. This interference introduces a significant dead time before data acquisition or the application of a subsequent radio-frequency pulse. The development of probes with actively shielded gradient coils has drastically reduced recovery times permitting the use of short and intense static gradients. Experiments enhanced with pulsed-field gradients have fewer artifacts, suffer far less from problems of solvents suppression and have reduced phase cycling requirements relative to the non-gradient experiments.

Applications:

A. Coherence Selection Coherence

Selection through the use of pulsed field gradients has many advantages. Unfortunately, the major disadvantage, a loss in sensitivity, can be significant, especially in the case of dilute biomolecules. To avoid this loss in sensitivity, pulse field gradients can be used to destroy magnetization which would otherwise lead to artifacts.

B. Artifact Suppression

Before development of gradient technology, artifacts in NMR experiments were eliminated by phase cycling i.e. by repeating the experiment several times with different phases for pulses and or the receiver. This process requires excellent spectrometer stability, often for a period of several days. In contrast, the use of PFG permits the suppression of artifacts on a per scan basis, there by reducing the demand for long-term spectrometer stability. This is particularly important, for example in heteronuclear applications involving unlabeled compounds.

C. Water Suppression

WATERGATE - WATER suppression by GrAdient tailored Excitation (2)

Magic angle gradients

Water flip-back pulses

D. Gradient Shimming

Software

Creating a field map

Shimming a sample

E. 3-Axis Gradients

ARE 3-axis gradients required or is a single-axis gradient sufficient? Most of the applications of gradients in high resolution spectroscopy have employed only a z-axis gradient probe. At NCSU we have 3-axis gradient probes. There are several reasons which make 3-axis gradient probes desirable. One of the best reason is ease-of-use, especially in complicated, multidimensional experiments.

References:

1. Lauterbur PC: Image formation by induced local interactions: examples employing nuclear magnetic resonance. Nature 1973, 242: 190-191.

2. Piotto M, Saudek V, Sklenar V: Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol .NMR 1992, 2: 661-665.

Additional Related Papers:

Kay, L. E,: Field Gradient techniques in NMR spectroscopy. Curr. Opin. in Struct. Biol.1995, 5:674-681. General review article.

Kay L.E, Pulsed Field Gradient Multi-Dimensional NMR Methods for the study of Protein Structure and Dynamics in solution: Prog. in Bioph. and Mol. Biol. 1995, 63(3):277-299.

Keeler J, Clowes RT, Davis AL, Laue E: Pulsed field gradients: theory and practice. In Methods in Enzymology, Nuclear Magnetic Resonance, Part C, Vol 239. Edited by James TL and Oppenheimer NJ. New York: Academic Press; 1994: 145-207. An excellent and current review of the theory and applications of pulsed-field gradients in high-resolution NMR

Hurd RE, John BK: Three-dimensional gradient-enhanced relay-edited proton spectroscopy. GREP-HMQC-COSY. J Magn Resonance 1991, 92: 658-668.

Vuister GW, Boelens R, Kaptein R, Burgering M, Van Zijl PCM: Gradient- enhanced 3D NOESY_HMQC spectroscopy. J Biomol NMR 1992, 2: 301-305.

Weigelt J,Otting G: 1H-Detected INEPT-INADEQUATE at natural ¹³C abundance. J Magn Resonance (A) 1995, 113: 128-130. Coherence transfer selection gradients are employed to select for ¹³C-¹³C double-quantum coherence allowing 10000-fold suppression of signals originating from ¹²C-bound protons.

Bax A, Pochapsky S: Optimized recording of heteronuclear multidimensional NMR Spectra using pulsed field gradient selection. J Magn Resonance 1992, 99: 638-643.

Muhandiram DR, Kay LE: Gradient-enhanced triple-resonance three-dimensional NMR experiments with improved sensitivity. J Magn Resonance (B) 1994, 103: 203-216.

Schleucher J, Schwendinger M, Sattler M, Schmidt P, Schedlezky O, Glaser SI, Sorensen OW, Griesinger C: A general enhancement scheme in hetronuclear multidimensional NMR employing pulsed field gradients. J Biomol NMR 1994, 4: 301-306.

WATERGATE experiment -WATER suppression by GrAdient Tailored Excitation.: Hwang, T. L., Shaka, A. J.: Water suppression that works. Excitation sculpting using arbitrary waveforms and pulsed field gradients. J Magn Resonance (A) 1995, 112: 275-279.

Grzesiek, S., Bax, A.: The importance of not saturationg H₂O in protein NMR. Application to sensitivity enhancement and NOE measurements. J Am Chem Soc 1993, 115: 12593-12594.

Kay, L. E., Xu, G. Y., Singer A, Muhandiram, D. R., Forman-Kay, J. D.: A gradient-enhanced HCCH-TOCSY expperiment for recording side chain ¹H and ¹³C correlations in H₂O samples of proteins. J Magn Resonance (B) 1993, 101: 333-337.

Wang, H., Zuiderweg, E. R. P.: HCCH-TOCSY spectroscopy of ¹³C-labeled proteins in H₂O using heteronuclear cross-polarization and pulsed-field gradients. J Biomol NMR 1995, 5: 207-211.

Pascal, S. M., Muhandiram, D. R., Yamazaki, T., Forman-Kay, J. D., Kay, L. E.: Simultaneous acquisition of ¹⁵N and ¹³C-edited NOE spectra of proteins dissolved in H₂O. J Magn Resonance (B) 1994, 103: 197-201.

Farrow, N. A., Muhandiram, D. R., Singer, A. U., Pascal, S. M., Kay, C. M., Gish, G., Shoelson, S. E., Pawson, T., Forman-Kay, J. D., Kay, L. E.: Backbone dynamics of a free and a phosphopeptide-complex Src homology 2 domain studied by NMR relaxation. Biochemistry 1994, 33: 5984-6003.

Altieri, A. S.,, Hinton, D. P., Byrd RA: Association of biomolecular systems via PFG-NMR diffusion measurements. J. Am. Chem. Soc. 1995, 117: 7566-7567.

Dotsch, V., Wider, G.: Exchange rates of internal water molecules in proteins measured using pulsed field gradients. J. Am. Chem. Soc. 1995, in press.

Warren, W. S. at al., Science 262. 2005-2009, (1993). DQF-COSY with magic Angle Gradients.

Introduction to Solid State NMR

Please note: Since the below was written, we have upgraded the Chemagnetics 200 MHz solid-state spectrometer and added a new Bruker DSX 300 MHz Wide Bore solid-state spectrometer to the Facility. We have also added a new faculty member, Jeffery L. White, whose web page will detail the current capabilities and directions of solid-state NMR in the Facility.

Review of Solid Polymer Characterization, Selected Articles, Book list

The following is an abstract of a presentation made in Angra dos Reis, RJ, Brazil on 13th May, 1997 at the 6th (semiannual) Encontro de Usários of the Associação de Usários de REssonância Magnética Nuclear (AUREMN), the Brazilian NMR Association, and published in that association's Annals. (Reproduced by permission.)

CHARACTERIZATION OF SOLID POLYMERS BY CP/MAS NMR
by
E. O. Stejskal
Department of Chemistry
North Carolina State University
Raleigh, NC 27695-8204
U. S. A.

Introduction

The application of NMR to the study of polymers has a long history. These studies have been directed to the examinationof both structure and molecular motion. Many of the earliest studies were performed on solid polymers and used low resolution techniques. However, many more studies have been made on polymer solutions and on rubbery polymers, using high resolution techniques.[1,2] More recently, with the development of techniques to obtain high resolution NMR spectra of rigid solids, these methods have been applied to glassy and crystalline polymers as well.[3,4]

There are two basic reasons to choose solid state NMR over solution NMR when studying rigid polymers. First there is the class of systems which cannot be put into solution with out changing them chemically. Cross linked polymers often fall into this class. Other polymers may be so fragile chemically that they decompose upon exposure to solvent. The second class of system that is better studied by solid state methods is not difficult to dissolve nor is it chemically fragile, but it would be changed physically if put into solution. A study designed to examine the physical properties of a solid polymer had better be done on the solid itself if it is to be relevant. There are many examples of relaxation studies made on solids polymers which succeed in examining molecular motion in the solid state.

Special techniques are required to obtain high resolution NMR spectra in the solid state. This is because rapid, isotropic molecular motion, which is responsible for simplifying so many nuclear interactions in the liquid state, is not present in sufficient amount in rigid solids. The special methods either supply the missing motion or remove the troublesome interactions. Depending on whether the spin system being studied is an abundant spin system with abundant internuclear couplings to be dealt with, or a rare spin system coupled primarily to a second spin system of abundant spins, different methods must be used to obtain high resolution NMR spectra. This overview will be principally concerned with the rare spin case, although some abundant spin methods and results will be discussed. We shall also be concerned primarily with natural abundance studies. Finally, unless stated otherwise, only spin one-half nuclei will be considered, most commonly ¹H and ¹³C.

Solid State NMR Methods

The most important solid state interaction to be dealt with, if we are to obtain high resolution spectra, is direct dipolar broadening of the object spin by its neighboring spins. In a study of rare spins, this broadening arises from the nearby abundant spins, frequently protons. It is easily removed by irradiating the abundant spins at their resonant frequency at sufficient rf power to overwhelm the abundant spin local fields.[5] This example of heteronuclear decoupling is simple and straightforward and only needs sufficient rf power. Homonuclear decoupling, as is required to remove dipolar broadening in a system of coupled abundant spins is not so straightforward.[6] It requires one of a number of irradiation schemes, either a train of pulses of carefully chosen length and phase, or suitably off-resonance cw irradiation. The observation of rare spins during heteronuclear decoupling is uncomplicated. Much greater instrumental demands result from the necessity to irradiate and observe the spin system simultaneously, as is required by the multiple-pulse homonuclear decoupling schemes.

Another barrier to high resolution in solids arises as a result of the absence of rapid, isotropic molecular tumbling: chemical shift anisotropy (CSA). The chemical shift associated with each different chemical environment is orientation dependent. For some spins in particularly anisotropic environments, the range of chemical shifts due to anisotropy is almost as large as the full range of isotropic chemical shifts normally seen for that spin. Overlapping CSA patterns obscure the isotropic shift information which is the usual object of an NMR experiment. The CSA is a problem for both rare spins and abundant spins. The solution is to substitute rapid sample rotation about a single axis for the missing molecular tumbling. The angle this axis makes relative to the laboratory magnetic field is called the magic angle, and the whole operation is usually called magic angle sample spinning (MAS).[7] The magic angle happens to turn out to be half the tetrahedral angle or, in other words, the angle the diagonal of a cube makes with any of its edges. A single rotation axis would not work if the CSA did not have a particularly simple angular dependence. For more complicated line broadening functions, such as quadrupolar interactions, techniques requiring rotation about two or more axes are required. Simultaneous high-speed rotation about two different axes has been achieved.[8]

Sensitivity is always a problem for rare spin NMR at natural abundance. It is even more a problem in the solid state where spin-lattice relaxation times, especially for spins without directly bonded abundant spins, can be exceptionally long. The usual approach to this problem is the third important technique used to obtain carbon, and other rare spin, NMR spectra in the solid state. Cross polarization (CP) is used to generate rare spin polarization by causing the rare spins to come to thermal equilibrium with the abundant spin system, which usually relaxes much more rapidly than the individual rare spins.[9,10] This process usually takes the form of a double rotating frame experiment and gives rise to a number of possible rotating frame relaxation experiments, which, as we shall see in the next section, are rich in information.

The combination of experiments described above for rare spins is usually called cross polarization/magic angle spinning (CP/MAS).[11] Abundant spins are observed with combined rotation and multiple pulse spectroscopy (CRAMPS).[12] Resolution is usually not as good in the solid state as that achieved in solution, even in crystalline systems. This is because there are still other sources of line broadening and limits to polarization lifetimes not seen in low viscosity liquids. In the glassy state, even less resolution is to be expected, because of the inherent disorder present in the glass. The isotropic chemical shift is sensitive to intermolecular as well as intramolecular interactions. Each slightly broadened line seen in the solid state spectrum is actually a band of isotropic chemical shifts reflecting the range of intramolecular interactions. Nevertheless, simple high resolution solid state spectra have been used with great effectiveness to answer basic questions about the primary chemical and physical structure in the solid state. The process of cross linking to produce an insoluble polymer can be readily followed as a solid state reaction. Non-equivalence of chemically equivalent atoms caused by restricted rotation in the solid state is also straightforward to detect.

Relaxation Phenomena

The cross polarization experiment has been described as a double rotating frame experiment. Both the abundant spin and the rare spin need to be spin-locked in an appropriate radiofrequency field if they are to communicate readily. The time constant for the loss of spin-locked polarization, usually characterized by the relaxation time T_1rho can be measured in both spin systems.[13] It behaves differently, however, in a rare spin system than in an abundant spin system. In an abundant spin system, T_1rho is a spin-lattice relaxation time sensitive to motion at frequencies double the precession frequency around the spin-locking radiofrequency field.[14] Furthermore, it is subject to spin diffusion, that is, spins within a certain distance of each other tend to relax cooperatively. Since this relaxation time can be measured through the rare spin system, it is possible to detect sample heterogeneity if different rare spins detect different groups of abundant spins that are not in good communication. Phase separation in polyblends and block copolymers can be detected in this experiment.

Spin diffusion does not operate effectively between well separated rare spins. Thus each rare spin relaxes at a rate indicative of its own special environment. Furthermore, rare spins may relax directly to the lattice and be sensitive to molecular motion, or relax to the abundant spin system and sense the strength of the coupling to that system.[15] Rare spin T_1rho spin lattice contributions are sensitive to motional frequencies near the precession frequency about the radiofrequency field. To use rare spin T_1rho as a detector of molecular motion, however, it is first necessary to determine the dominant relaxation mechanism.

Line Shape Analysis

The techniques by which high resolution spectra are obtained in the solid state require the suppression of the dipolar coupling and the averaging away of the CSA. The result is the loss of considerable information. There are ways to recover this lost lineshape information. The CSA information can be recovered by spinning slowly enough to leave spinning sidebands in the spectrum from which the lost CSA lineshape can be inferred by a suitable mathematical analysis.[16] If overlap between spinning sidebands and centerbands proves too complicated to interpret, two-dimensional methods exist which separate the spinning sidebands from the isotropic shifts.[17] The most straightforward way to recover the lost dipolar lineshape information in a rare spin NMR experiment is to perform a two-dimensional NMR experiment in which the dipolar coupling is restored during the t₁ evolution period. The data analysis is much clarified if the abundant spins are decoupled from one another while they are allowed to couple to the rare spins. There are a variety of these separated local field (SLF) experiments in current use.[18,19] The lineshape information obtained from these experiments can be combined with relaxation time measurements to characterize both rates and amplitudes of molecular motion. Particularly, molecular motion will leave its mark on a detected lineshape by the extent to which it averages the lineshape expected for the interaction in the absence of motion.[20]

As normally analyzed, the CSA is assumed to arise from a crystal powder average of molecular orientations. Just as an anisotropic motion can average the CSA to an unusual lineshape, an oriented sample will yield an atypical lineshape. Several different techniques have been developed to characterize oriented samples and to calculate lineshapes.[21]

Phase Separation

Solid polymers have been characterized extensively by Solid-State NMR methods. Spectral information, linewidths, and relaxation studies give direct information about structure and motion in the solid-state. However, even the most ordered polymers possess a certain amount of heterogeneity, which often affects the properties of the material. Blends of compatible or incompatible polymers, block copolymers, and multiphase systems present even greater tendencies to develop domains of unknown size, which need to be understood to understand the mechanical properties of the solid.[22,23]

One class of NMR experiments which has the potential to determine domain sizes and differences in properties between domains involves the determination of spin diffusion. The time it takes for spin information to cross from one domain to another will tell the size of the domain if the rate of spin diffusion is known. Diffusion is usually measured by setting up some kind of non-uniform concentration gradient and then watching it dissipate. In this case, the concentration gradient is spin polarization or other spin property. In the classical Goldman-Shen experiment, suppression of the signal from one of two communicating systems with a well-enough resolved chemical shift difference was based on differences in precession rate alone.[24] Restoration of equilibrium between the two systems gave information about the rate of communication between them. (This original experiment was all the more remarkable for having been done on a phase-incoherent spectrometer!)

Some spatial information can also be gotten from multi-phase systems which develop their own gradients by means of unequal relaxation rates within the phases.[23] However, in most common, less cooperative cases, it is necessary to enhance the resolution between the parts of the system to be separated before undertaking to prepare gradients by perturbing the population (selective excitation or suppression) of part of the system.[25-27] 2D methods are often useful in the preparation of the system and resolution of the data in these cases.[4,28,29] Spin diffusion studies usually focus on the abundant spin system because spin diffusion is too slow in the rare spin system if labels are not used. For that reason multiple pulse decoupling (often combined with magic angle sample spinning as CRAMPS) is usually the preferred method of enhancing the resolution between parts of the system. It also allows control of communication between abundant spins.[6] Whether the abundant spin or a rare spin ultimately is observed depends on which high resolution spectrum is most sensitive to the effects of spin diffusion.

Conclusions

As in the early days of low resolution solid state NMR, many of the applications of high resolution solid state NMR have been to polymers. It has been used to study solid state reactions, chemical and physical structure, phase separation, and extensively to characterize the frequency, amplitude and particular axes of molecular motion. The basic NMR techniques responsible for high resolution: decoupling, magic angle sample spinning, and cross polarization were early developments in the science of NMR. New approaches to amplify these methods and make them more useful are being developed every day.

References
1. Bovey, F. A., "High Resolution NMR of Macromolecules," Academic Press, New York, 1972.

2. Bovey, F. A. and Jelinski, L. W., "Chain Structure and Conformation of Macromolecules," Academic Press, New York, 1982.

3. McBrierty, V. J. and Packer, K. J., "Nuclear Magnetic Resonance in Solid Polymers," Cambridge University Press, Cambridge, 1993.

4. Schmidt-Rohr, K. and Spiess, H. W., "Multidimensional Solid-State NMR and Polymers," Academic Press, Ltd., London, 1994.

5. Bloom, A. L. and Shoolery, J. N., "Effects of Perturbing Radiofrequency Fields on Nuclear Spin Coupling," Phys. Rev. 97, 1261 (1955).

6. Waugh, J. S., Huber, L. M., and Haeberlen, U., "Approach to High-Resolution NMR in Solids," Phys. Rev. Lett. 20, 180 (1968).

7. Andrew, E. R., Bradbury, A. and Eades, R. G., "Removal of Dipolar Broadening of Nuclear Magnetic Resonance Spectra of Solids by Specimen Rotation," Nature 183, 1802 (1959).

8. Chmelka, B. F., Mueller, K. T., Pines, A., Stebbins, J., Wu, Y., and Zwanziger, J. W., "Oxygen-17 NMR in Solids by Dynamic-Angle Spinning and Double Rotation," Nature 339, 42 (1989).

9. Hartmann, S. R. and Hahn, E. L., "Nuclear Double Resonance in the Rotating Frame," Phys. Rev. 128, 2042 (1962).

10. Pines, A., Gibby, M. G., and Waugh, J. S., "Proton Enhanced NMR of Dilute Spins in Solids," J. Chem. Phys. 59, 569 (1973).

11. Schaefer, J. and Stejskal, E. O., "Carbon-13 Nuclear Magnetic Resonance of Polymers Spinning at the Magic Angle," J. Am. Chem. Soc. 98, 1031 (1976).

12. Jackson, P. and Harris, R. K., "A Practical Guide to Combined Rotation and Multiple-Pulse NMR Spectroscopy of Solids," Magn. Reson. in Chem.26, 1003 (1988).

13. Schaefer, J. Stejskal, E. O., and Buchdahl, R., "Magic Angle ¹³C Analysis of Motion in Solid Glassy Polymers," Macromolecules10, 384 (1977).

14. Jones, G. P., "Spin-Lattice Relaxation in the Rotating Frame: Weak Collision Case," Phys. Rev. 148, 332 (1966).

15. Schaefer, J., Sefcik, M. D., Stejskal, E. O., and McKay, R. A., "Carbon-13 T_1rho Experiments on Solid Polymers Having Tightly Spin-Coupled Protons," Macromolecules 17, 1118 (1984).

16. Herzfeld, J. and Berger, A. E., "Sideband Intensities in NMR Spectra of Samples Spinning at the Magic Angle," J. Chem. Phys.73, 6021 (1980).

17. De Lacroix, S. F., Titman, J. J., Hagemeyer, A., and Spiess, H. W., "Increased Resolution in MAS NMR Spectra by Two-Dimensional Separation of Sidebands by Order," J. Magn. Reson. 97, 435 (1992).

18. Schaefer, J., McKay, R. A., Stejskal, E. O., and Dixon, W. T., "Dipolar Rotational Spin-Echo ¹³C NMR of Polymers," J. Magn. Reson.52, 123 (1983).

19. Munowitz, M. G. and Griffin, R. G., "Two-dimensional Nuclear Magnetic Resonance in Rotating Solids: An Analysis of Line Shapes in Chemical Shift-dipolar Spectra," J. Chem. Phys. 76, 2848 (1982).

20. Schaefer, J., Stejskal, E. O., McKay, R. A., and Dixon, W. T., "Molecular Motion in Polycarbonates by Dipolar Rotational Spin Echo C-13 NMR," Macromolecules17, 1479 (1984).

21. Harbison, G. S. and Spiess, H. W., "Two-Dimensional Magic-Angle-Spinning NMR of Partially Ordered Systems," Chem. Phys. Lett. 124, 128 (1986).

22. Stejskal, E. O., Schaefer, J., Sefcik, M. D., and McKay, R. A., "Magic-Angle Carbon-13 Nuclear Magnetic Resonance Study of the Compatibility of Solid Polymeric Blends," Macromolecules 14, 275 (1981).

23. Schaefer, J., Sefcik, M. D., Stejskal, E. O., and McKay, R. A., "Magic-Angle Carbon-13 Nuclear Magnetic Resonance Analysis of the Interface between Phases in a Blend of Polystyrene with a Polystyrene-Polybutadiene Block copolymer," Macromolecules 14, 188 (1981).

24. Goldman, M. and Shen, L., "Spin-Spin Relaxation in LaF₃," Phys. Rev. 144, 321 (1966).

25. Caravatti, P., Levitt, M. H., and Ernst, R. R., "Selective Excitation in Solid-State NMR in the Presence of Multiple-Pulse Line Narrowing," J. Magn. Reson. 68, 323 (1986).

26. Schmidt-Rohr, K., Clauss, J., Blümich, B., and Spiess, H. W., "Miscibility of Polymer Blends Investigated by ¹H Spin Diffusion and ¹³C NMR Detection" Magn. Reson. in Chem.28, S3 (1990).

27. Campbell, G. C. and VanderHart, D. L., "Optimization of Chemical-Shift-Based Polarization Gradients in ¹H NMR Spin-Diffusion Experiments of Polymer Blends with Chemically Similar Constituents," J. Magn. Reson.96, 69 (1992).

28. Schmidt-Rohr, K., Clauss, J., and Spiess, H. W., "Correlation of Structure, Mobility, and Morphology by 2D WISE NMR," Macromolecules25, 3273 (1992).

29. Clauss, J., Schmidt-Rohr, K., and Spiess, H. W., "Determination of Domain Sizes in Polymers," Acta Polymer. 44, 1 (1993).

Click for the Solid State NMR reading list in pdf format
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NMR of RNA

The NMR Facility Staff serves the facutly of the Biotechnology Program.

RNA Society of North Carolina

NMR has moved a long way...

Biomolecular nuclear magnetic resonance (NMR) has advanced rapidly ( Methods in Enzymology Vol 239 and 261 ) since the publication in 1989 of the Methods in Enzymology volumes 176 and 177. Much progress has resulted from generalization of heteronuclear two-dimensional (2D) NMR experiments with ¹³C and¹⁵N labeled biomolecules to higher dimensions. Three- and four- dimensional techniques, although straightforward conceptual elaborations of two- dimensional NMR, have proved to be powerful tools to the structural biochemist. The development of sophisticated pulse sequences has driven the development of more elaborate instrumentation ( and vice versa).
Structure determination via NMR is distinctly different from the situation with X-ray crystallographic determination of structure, yielding a single most favorable structure. In contrast with the NMR an effort is made to generate an array of structures which are consistent with the available NMR data in order to define the limits of the structure. It is desirable to make the array of structures large enough to map out the conformational space which will accommodate the available experimental data.

NMR spectroscopy is currently the only method available for the high-resolution solution structure determination of the biomolecules (23-25).

Structure determination by NMR

1. Collect the experimental data

2. Process the data

3. Determine structural constraints

4. Calculate structures which satisfy these constrains

Reviews of the steps involved in Protocol 1 are given by, Wuthrich
Prior to 1982, assignment of resonances in the complex NMR spectra of proteins was achieved using 1D NMR, and was based, for the most part, on the assumption that the structure of the protein in solution was the same as in the available X-ray structure. The introduction of 2D NMR techniques such as COSY and NOESY in the early 1980s dramatically increased the resolution of protein NMR spectra and as a result the amount of information which could be obtained from the spectra also increased. In 1982 Wuthrich and co-workers introduced a systematic method for the full assignment of the 2D NMR spectra of proteins which relied only on information about the amino acid sequence of the protein; this method is the sequential assignment procedure (1,2). This approach is now used by most researchers who apply NMR spectroscopy to the study of proteins.

NMR Studies of RNA. NMR spectroscopy is the method of choice to study details of the solution structure of nucleic acids (23-25). Known motifs observed in RNA (30) include: double helical stems, hairpin loops (31-33a-c), internal loops, bulges (34a-c), mismatched base pairs (35), multi-stem junctions, base triples (36-38), and pseudoknots (39a,b). The specific shape as well as the hydrogen-bonding properties of RNA will contribute to the recognition between RNA and proteins (22). Understanding the structure, the dynamics and the thermodynamic stability of variety of structural motifs in the IRE will add to understanding of the principles of RNA folding, the specificity of RNA protein interactions, and functional features of mRNA.
Nucleic acids are perhaps the most intensively studied molecules found in nature, which is not surprising in view of their role as carriers of genetic information. It has been a deep-rooted belief among molecular biologist that structure dictates function and, therefore, these studies have often involved the elucidation of structural aspects of these molecules.. In a similar spirit it has been a deep-rooted belief among NMR spectroscopists that "their" field of spectroscopy should be able to contribute to the elucidation of the structure and dynamics of molecules, in particular, biomolecules, in a way unparalleled by other forms of spectroscopy. Over the last few years this goal has come within reach thanks to the development of multidimensional NMR techniques which provide the necessary spectral resolution for the successful interpretation of the complicated spectra of biomolecules. Triggered off by the development of two-dimensional NMR, an avalanche of NMR studies on nucleic acids has appeared (1). Methods for spectrum interpretationand structure elucidation have been developed.

1. Helical structures, Hairpin Loops, Internal Loops and Single Base Bulges. Hairpin loops are important structural elements providing sites for interactions with proteins and other nucleic acids and serving as nucleation sites for RNA folding (31,39). Despite this fact, relatively little detailed information is available regarding such RNA structures even in the most recent NMR studies of 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage. R17 structure has not been precisely defined. Most of what is known has been derived from X-ray structure of tRNAs and small duplex oligonucleotides (39).

Recently, NMR spectroscopy has been applied to a variety of small RNA molecules; solution conformation of several RNA hairpins have been reported (31). The 3D structure of individual RNA hairpins is highly variable. Although stems are generally A form, local perturbations due to sequence or structural context are common. Loop conformations vary over a wide range.

Thermodynamic studies on RNA hairpin loops showed that stability depends on the formation of hydrogen bonds between base pairs in the loop (30-34,37). Most recently the three dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics and energy minimization (31). Although the loop structure is still tentative, the known interactions sites with coat protein are easily accessible from the major groove side of the loop. Base pairing in the hairpin loop appears to occur in the IRE deduced from the poor reactivity of G18 and C14 to RNAase T₁ and dimethyl sulfate, respectively (15-18; Fig. 1) and the decrease in temperature stability of both the main helix (T_m=10°) and specific features of the imino environment in the 1H spectrum of the G18A mutant (Fig. 6; 1).

The stem of the IRE hairpin is interrupted by a single-base bulge on the 5' side for the five transferrin receptor IREs (a-e) and the eALAS (erythroid aminolevulinate synthase) IRE. A four-nucleotide internal loop characterizes the stem of the ferritin IRE with three nucleotides, UGC, on the 5' side and C on the 3' side. Internal loops and bulges have been previously examined by NMR models for 5S ribosomal RNA (40a-b,41) and the TAR element in HIV viruses (27a-d,42). For example, 2D NMR analysis of the shorter sequence of the E loop from 5S ribosomal RNA of X. laevis (n=27), followed by restrained molecular dynamics showed that the highly conserved internal loop closes to form a G/A base pair and a reverse Hoogsteen A/U base pair; the resulting conformation suggested the possibility of a base triple (36,37). The conformation and dynamics of an RNA oligonucleotide (n=25 nucleotides), which is a model of the tetrahymena group I intron, has also been investigated by one- and two-dimensional NMR spectroscopy (37,38); the exchangeable proton spectrum indicated that two helices stack coaxially with adjacent single-stranded nucleotides forming base triple.

NMR spectroscopy has also been used to analyze the effect of single base bulges on intercalation (34). In these hairpins, single-base bulges seem to strongly facilitate changes to alternate conformations. It seems likely that the functional role for single-base bulges and internal loops in RNAs may be to permit the RNA to switch between different conformations (27d). In this context it is useful to recognize that the internal loop region of the ferritin IRE, which interacts with both initiation factors plus the IRP, has alternate conformations detected by probing with 1,10-phenanthroline-Cu (21).

2. Folded helical structures: Pseudoknots and Other Higher Order Interactions. Higher order interactions of the IRE are likely to be responsible for the folding and unusual reactivity observed with protein nucleases, transition metal complexes (3,4,15,16) and the NMR spectrum of the wild-type compared to the hairpin loop mutant (G18A) IRE sequences (Figs. 5 and 6). Base-triples (36-38), pseudoknots (39a-c) and coaxially stacked helices (38,43) are among essential tertiary interactions in RNA three-dimensional structure.

RNA pseudoknots are found in virtually all classes of RNA and play key roles in a variety of biological processes. Pseudoknots formation folds the 3’ ends of many plant viral genomic RNAs into structures that resemble transfer RNA in global folding and in their reactivity to transfer RNA-specific proteins. The solution structure of the 44- nucleotide pseudoknotted T arm and acceptor arm of the transfer RNA-like structure of turnip yellow mosaic virus (TYMV) was determined at an atomic level in all regions by nuclear magnetic resonance (NMR ) spectroscopy. The structure resulting from this interaction between the minor groove and single -stranded RNA at helical junction display internal mobility, which may be a general feature of RNA pseudoknots that regulates their interaction with proteins or other RNA molecules

The hammerhead, a RNA oligomer of 40 nucleotides with tertiary interactions, is fully active in the self-cleavage reaction (44). Complete assignment of low-field oligomer ring imino protons between adjacent pairs has led to NOEs indicating a pseudoknot (39a-c). Formation of a pseudoknot in the corresponding oligomer is shown by the existence of two helical stem regions deduced from NOEs between the imino protons. The two-stem regions stack to form a continuous helix with a possible minor distortion in helical stacking at the junctions of stems and loops (39a-c,43). tRNA, the RNA paradigm for 20 years (28a-c), also illustrates how the deduction of stacked helices and junction distortions (16) is made from NMR spectroscopy (38-40). In addition, the results of NMR spectroscopy of tRNA in solution confirmed the L-shape previously observed in crystals (28c).

3. NMR analysis of tRNA
The structure-function relationships of RNAs, such as that of tRNA in protein synthesis, are fundamental to all cell and molecular biology, important as tools of science and targets of opportunity for pharmaceuticals.
Transfer RNA is actually a large set of molecules which we tend to speak of as the generic tRNA structure, and for which one of the most common features are the 80 modified nucleosides. The biophysical aspects of tRNA function are relatively unknown. Study of tRNA utilizes molecular genetic, microbiological, biochemical, and chemical methods to more clearly and precisely define the site-specific structure-function relationships. We have found that modified nucleosides in tRNA play an important structural and functional role both within the tRNA molecules and in tRNA anticodon recognition of select codons at the wobble position resulting in a modified wobble hypothesis. we have proven that the tight binding of metal ions to RNA is correlated with modification of the RNA sequence. Modified nucleosides facilitate metal ion interactions with RNA resulting in micromolar binding constants and added stability to the structure. Metal ions play important roles in the structural dynamics of the RNA molecule and these structural changes are functionally important. With an understanding of how modified nucleosides facilitate metal ion binding to the anticodon stem and loop of yeast tRNA^Phe, we have designed a DNA analogue that effectively inhibits tRNA^Phe from binding the ribosome, and inhibit protein synthesis in vitro and in vivo.

RNA Structure Reported until 1998

Name	Method	Authors of Reference
Iron responsive element hairpin loop	NMR	Laing andHall, 1996; Addess, K.J., Basilion, J.P. Klausner, R.D., Rouault, T.A., and Pardi, A.,(1997); Gdaniec, Z., Sierzputowska-Gracz, H., Theil, E.C.,(1997);
Hepatitis delta central hairpin	NMR	Lynch, S., and Tinoco, I., Jr.; Kolket al., 1997
TYMV pseudoknot	NMR	Kolk, M.H., van der Graaf, M., Wijmenga, S.S., Pleij, C.W.A., Heus, H.A., and Hilbers, C.W.
SRP domain IV hairpin	NMR	Lukavsky et al., 1997
Spliced leader RNA	NMR	Xu, J., and Crothers, D.M.
16SrRNA A site-paromomycin complex	NMR	Fourmy et al., 1996
16SrRNA A site-gentamicin complex	NMR	Yoshizawa, S., Fourmy, D., and Puglisi, J.D.
16S ribosomal A site	NMR	Fourmy, D., Yoshizawa, S., Puglisi, J.D.
ATP-binding RNA aptamer	NMR	Dieckmann et al., 1996
Tetraloop receptor RNA	NMR	Butcher, S.E., Dieckmann, T., and Feigon, J.
UGAA tetraloop	NMR	Butcher et al., 1997
Gene 32 mRNA pseudoknot	NMR	Du et al., 1996
16S rRNA binding site for protein S8	NMR	Kalurachchi et al., 1997
Rev peptide-RRE RNA complex	NMR	Battiste et al., 1996
Leadzyme	NMR	Hoogstraten, C.G., Legault, P., and Pardi, A.
Theophylline-binding RNA aptamer	NMR	Zimmermann et al., 1997
Group I ribozyme P5b hairpin loop	NMR	Kieft and Tinoco, 1997
Sarcin/ricin loop	NMR	Szewczak et al., 1993
U2 snRNA stem loop IIA	NMR	Stallings, S., and Moore, P.B.
3' terminal helix in 16S	NMR	Rife, J., and Moore, P.B.
42 nt fragment from 5S	NMR	Dallas, A., and Moore

4. RNA/Protein Interactions. RNA/protein interactions (22,50) studied by NMR (51) spectroscopy include Tat/TAR) (27a-c), polycistronic mRNA/viral coat proteins (46-48), 16S RNA/sarcin ribosomal protein S5 (49) and S17 ( ) and ricin ribosomal protein ( ).

The interaction of the TAR stem/loop in HIV RNA with the regulatory protein called Tat activates the transcription of the RNA. The stem in TAR is interrupted by a three-residue bulge in the middle of the 5'-side of the stem. The bulged residue -U23, C24 and U-25 changed conformation when Tat protein binds or when a small basic peptide from Tat binds, or even when free arginamide binds, thus, permitting examination of the RNA structure in the simple complex with arginamide (27a-e). In the absence of arginamide, the three residues in the bulge stack on top of each other, continuing the strand to which they belong in a way that accounts for the observation that the stem as a whole is bent. Arginamide hydrogen bonds to the phosphate groups that link A22 to U23 and U23 to C24 as well as to hydrogen-bond acceptors belonging to the guanine of the GC immediately above the bulge. This appears to position U23 so that it can form a base-triple with the AU immediately above the GC pair adjacent to the bulge which, in turn, causes C24 and U25 to turn outward into solution. Among other things, this conformational change straightens the stem. The importance of the AUU base-triple, which is the most distinctive feature of the model, has been verified by site-directed mutagenesis (27e). The advantage of introducing selectively enriched nucleotides was illustrated first for a TAR sequence (42) by using the ¹³C chemical shift to provide an alternative method to determine ribose puckers in large RNAs (52,53).

5. Affinity and Specificity
Proteins bind RNA with the affinity required for complex formation at the concentrations of reagents present in living cells and for regulation of biological function. For example, transfer RNA (tRNA) synthetase enzymes bind tRNA with modest affinity (the bimolecular dissociation constant K_d= 10^-6 M): these enzymes would be ineffective if the substrate was not released after catalysis. In contrast, components of RNA-protein complexes that are permanently assembled (such as the human U1A protein) bind cognate RNAs much more tightly (K_d< 10^-9 M). The analysis of the few existing structures of RNA-protein complexes determined at atomic resolution reveals that affinity is not simply related to parameters such as protein charge or the size of intermolecular interface area. For example, tRNA-synthetase complexes have very large interface areas but bind RNA weakly, while the human U1A protein, with a small interface area, binds RNA very tightly.
Understanding the molecular determinants of the binding energy of an RNA-protein interaction is a very important but insufficient goal: specificity is equally important. Many RNA-binding proteins bind any RNA weakly, regardless of its sequence or structure. However, biological function requires discrimination of cognate RNAs (the correct, relevant targets) from noncognate RNAs, which are present in very large excess in the cell. The difference in binding energy between cognate and noncognate RNAs defines how specific an RNA-protein interaction is. Understanding this specificity adds a further, intriguing dimension to the characterization of these

RNA MOLECULE

RNA molecules play a central role in all the main functions of living molecules: storage of genetic information, propagation of the genetic material, and enzymatic activity. RNA molecules do not perform those functions alone, but in tight associations with RNA-binding proteins. Thus, RNA-protein recognition is central to understanding a wide range of biological processes.
RNA-protein recognition cannot be understood with out recognizing that the diversity and complexity of RNA structures are comparable to those of proteins. This structural diversity defines an enormous variety of sites and shapes for intermolecular recognition..

6. Molecular modeling as a tool to find 3D structures of RNA: The analysis of RNA 3-D structures is crucial for understanding the structure-function relationship and evolution of RNA. Molecular modeling aims to find the 3-D structures of RNA that satisfy the structural information obtained by various methods such as electron microscopy, neutron scattering, low resolution X-ray and NMR analysis, site-directed mutagenesis, crosslinking, chemical and biochemical probing, phylogenetic comparison and secondary structure prediction by free energy minimization (27c-d,31,53-55). A current example of the application of NMR analysis and modeling is the RNA binding sequence IRE.
The IRE and IRE/IRP complex. The IRE family is the best characterized group of eukaryotic mRNA regulatory elements and is one of the longest conserved RNA sequences known (n=28-30). A hairpin loop with higher order interactions has been indicated for the IRE by comparing wild-type ferritin mRNA to RNA with altered IRE sequences, using classical protein nucleases, an alkylating reagent and shape selective transition metal complexes as probes (15-18,21); interactions as far away as 20 nucleotides upstream from the IRE have been observed using site-directed mutation and Fe-bleomycin as a probe (17). The results have been related to mRNA function measured as ferritin synthesis in vitro (17,20).

References...coming soon on separate page

Last modified by aobw August 2002.