Nucleic Acids Facility (NAF) at North Carolina State University (NCSU)
Nucleic Acids Facility (NAF) at North Carolina State
Critical to a project are proper planning and design of each oligo. To ensure client satisfaction, NAF devotes time and effort to these two aspects in the beginning stages of each client's project. In this manner NAF gains a complete understanding of the project's purification requirements, assay detection methods and scale-up issues.
NAF successes have been based on identifying and addressing challenges presented throughout the client's project. We at NAF want to understand why an oligo performs as required as well as why, if it does not. In this manner our motto and worth are fulfilled. NAF considers your oligo order complete only when it has contributed to the progress of the project for which it was intended.
Synthesis Nucleic Acids Facility (NAF) at North Carolina State University (NCSU)
NAF offers custom oligos with modifications in site specific introduction as our specialty. Synthesizing oligonucleotides with chemicals as opposed to enzymes ensures certainty of the following aspects.
- Site specific incorporation of modified units
- Exact sequence produced
- Large volumes of full-length product
Chemical Synthesis of Oligonucleotides Offered by the Nucleic Acids Facility
Scales of Synthesis
DNA, RNA, & DNA-RNA chimeras, 200nM & 1µM
Special Orders from 1µM to 15µM
Oligo Length: Number of BasesDNA - 1 to 125Yield
RNA - 1 to 45 (more than 45 under special circumstances)
DNA-RNA chimeras - 1 to 80, limit of 45 for the RNA component
(more than 80 under special circumstances)Sequence, length of oligo and scale of synthesis can vary the expected yield. The following estimates of yield are generalizations with no guarantee.DNA 200nM scale (17 to 20 mer) yields 1mg DNA
1µM scale yields 2mg DNA
RNA 200nM scale (17 to 20 mer) yields (fill in later)
1µM scale yields 600 to 800 µg
Site specific incorporation of modified nucleosides is one advantage of chemical over enzymatic synthesis.
At NAF we work with commercially available monomers, and will also consider working with groups preparing monomers that are "home made."
Examples of modifiers used by NAF are -Glen Research Corporation <http://www.glenres.com>Fluorescein 2' - O Me - RNAChem Genes Company <http://www.chemgenes.com>
Biotin - TEG 2' - NH ....RNA
Getting Started Menu
Please - Read this first.
Providing specialty oligos is usually an experimental adventure due to the non-standard nature of each user's needs... and begins with personal contact.Your Oligo RequirementsTo initiate your DNA or RNA synthesis order, contact the facility either by telephone or Email with the following information, to clarify your requirements.Yield
Level of Purity
How We Can Contact YouEmail Address
Getting Started Menu