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Zeiss LSM 710 confocal workstation

The Zeiss LSM 710 is a state of the art laser scanning confocal microscope that incorporates the latest advances in optics, lightpath efficiency, low noise scan electronics and detectors. This instrument has advanced imaging capabilities offering high sensitivity with superior signal to noise ratio, spectral analysis with a 34 channel detector and the ability to monitor dynamic changes of multiple fluorophores. Modules for advanced confocal applications such as Fluorescence Energy Transfer (FRET), colocalization analysis, and Recovery After Photobleaching (FRAP) are available. The multi-time plug in allows automated 3D (x,y,z) or 4 D (x,y,z,t) scans of preprogrammed locations and 3D image stiching. Funding was provided in part by the National Science Foundation (MRI-0821192), the College of Agriculture and Life Sciences, the NCSU Biotechnology Program and the NCSU Office of the Vice Chancellor for Research.

Laser lines for excitation:

• 405 nm diode laser (for DAPI, ALexa 350)
• 458 nm, 488 nm, 514 nm multiline argon laser
• 561 nm diode pumped solid state laser
• 633 nm HeNe laser

Emission windows can be flexibly selected with 2 flanking photomultiplier (pmt) channels and a 34 channel pmt array. The system can be opperated in lambda mode with 34 spectral detectors that collect the entire wavelength range in one scan or in channel mode with up to 10 simultaneously active detection channels that can be set as needed.

Differential interferrence contrast images can be acquired simultaneously and DIC prisms are available for all objectives except 5x.

Microscope and Accessories: The scanhead is attached to a Zeiss Axio Observer Z1 inverted microscope with motorized, programmable x,y,z stage, joystick and touch panel docking control station. A definite focus attachment corrects for focus drifts during overnight time lapse experiments. Temperature and CO2 control chambers are available.

Objectives:

Check out the Zeiss educational site to learn more about objective specifications. More information about the instrument can be found on the Zeiss webpage (click links below).

• LSM 710
• Axio Observer