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Zeiss LSM 710 confocal workstation

The Zeiss LSM 710 is a state of the art laser scanning confocal microscope that incorporates the latest advances in optics, lightpath efficiency, low noise scan electronics and detectors. This instrument has advanced imaging capabilities offering high sensitivity with superior signal to noise ratio, spectral analysis with a 34 channel detector and the ability to monitor dynamic changes of multiple fluorophores. Modules for advanced confocal applications such as Fluorescence Energy Transfer (FRET), colocalization analysis, and Recovery After Photobleaching (FRAP) are available. The multi-time plug in allows automated 3D (x,y,z) or 4 D (x,y,z,t) scans of preprogrammed locations and 3D image stiching. Funding was provided in part by the National Science Foundation (MRI-0821192), the College of Agriculture and Life Sciences, the NCSU Biotechnology Program and the NCSU Office of the Vice Chancellor for Research.

  • 405 nm diode laser (for DAPI, ALexa 350)
  • 458 nm, 488 nm, 514 nm multiline argon laser
  • 561 nm diode pumped solid state laser
  • 633 nm HeNe laser

Differential interferrence contrast images can be acquired simultaneously and DIC prisms are available for all objectives except 5x.

Microscope and Accessories: The scanhead is attached to a Zeiss Axio Observer Z1 inverted microscope with motorized, programmable x,y,z stage, joystick and touch panel docking control station. A definite focus attachment corrects for focus drifts during overnight time lapse experiments. Temperature and CO2 control chambers are available.

Magnification

N.A. Immersion

Type

notes

5 x

0.16 dry

Plan-Apochromat

On request

10 x

0.45 dry

Plan-Apochromat

 

20 x

0.8 dry

Plan-Apochromat

 

25 x

0.8 W/oil/glycerin

Plan-Apochromat

On request

40 x

0.75 dry

Plan-Neofluar

On request

40 x

0.95 dry

Plan-Apochromat

On request

40 x

1.1 W

C-Apochromat

 

40 x

1.3 oil

Plan-Neofluar

 

63 x

1.4 oil

Plan-Apochromat

 

63 x 1.2 W C-Apochromat  

Check out the Zeiss educational site to learn more about objective specifications. More information about the instrument can be found on the Zeiss webpage (click links below).

 

 


Monday July 28, 2014

Director:
Dr. Eva Johannes
(919) 515 3525
johannes@ncsu.edu

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Page last updated on April 7, 2014 by EJ