Imaging Facility
Box 7612
Leica TCS SP1 confocal workstation
The confocal scanhead is normally attached to an inverted Leica IMBE microscope and can also be used on an upright Leica RXA microscope. A custom-built cradle is available for sideways operation of the Leica RXA for gravity research. Both microscopes are equipped with prisms for differential interference contrast (DIC) and have a camera port with c-mount adapter.
- 351 and 364 nm UV (Coherent, EnterpriseII 651)
- 488 and 514nm Argon (Uniphase)
- 561 nm Yellow diode-pumped solid state laser (Melles Griot 85-YCA)
- 633 nm HeNe
The emission window of the 3 photomultipliers can be set to match the spectral properties of the fluorescent label. DIC images can be recorded simultaneously using the transmitted light detector and appropriate prisms. A free reader with basic data processing that runs on Windows XP can be obtained from the Leica ftp site (right click here and save installation file on your hard drive).
Leica IMBE (inverted)
- DIC for 10x, 20x, 40x, 63x, 100x objectives
- standard filter cubes for FITC, TRITC, DAPI
- galvo z-stage
- motorized turret and z-control through RS port
Leica RXA (upright)
- standard filter cubes for FITC, TRITC, DAPI
- rotating stage
- support cradle for sideways operation (gravity research)
- motorized z-control through RS port
Magnification |
N.A. Immersion |
Type |
notes |
5 x |
0.13 dry |
Plan Fluotar |
IMBE |
10 x |
0.3 dry |
Plan Fluotar |
On request |
20 x |
0.6 dry |
Plan Apochromat |
IMBE |
20 x |
0.45 dry |
Plan Fluotar Pol |
On request |
40 x |
1.25-0.75 oil |
Plan Apochromat |
IMBE |
40 x |
0.55 dry |
N Plan Corr |
On request |
63 x |
1.2 water |
HCX Plan Apochromat Corr |
IMBE |
100 x |
1.4-0.7 oil |
Plan Apochromat |
IMBE |
100 x |
1.3-0.6 oil |
FL Fluotar |
On request |
Thursday May 23, 2013
Interim Director:
Dr. Eva Johannes
(919) 515 3525
johannes@ncsu.edu
For new users
Facility policy
Price list
Facility news
