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Imaging Facility
actin in onion nucleus
NCSU North Campus4115 Gardner Hall Box 7612ncsu logo


Zeiss Lightsheet Z.1 Workstation

This microscope is ideal for multiview and long term live imaging of medium to large transparent specimen. The specimen is embedded in low melting point agarose and suspended in a medium-filled environmental chamber. A sheet of laser light is created using 2 illumination objectives that are positioned perpendicular to the detection objective. This way only a narrow plane of the specimen is illuminated. Optical sectioning is achived by moving the specimen relative to the illumination and detection optics. Images are acquired with 2 s-CMOS PCO.edge cameras.

 

  • 405 nm 50 mW
  • 445 nm 25 mW
  • 488 nm 50 mW
  • 515 nm 20 mW
  • 561 nm 50 mW
  • 638 nm 75 mW
  • DAPI - GFP (beam splitter SBS LP 490; emission filters BP 420-470 and BP 505-545)
  • CFP - YFP (beam splitter SBS LP 510; emission filters BP 460-500 and BP 525-565)
  • GFP - mCherry (beam splitter SBS LP 560; emission filters BP 505-545 and LP 585)
  • GFP - DRAQ5 (beam splitter SBS LP 560; emission filters BP 505-545 and LP 660)
  • Universal green-red ((beam splitter SBS LP 560; emission filters SP 550 and LP 585)
  • 5x 0.16 NA (dry)
  • 20x 1 NA (water dipping)
  • 40x 1 NA (water dipping)
  • zebrafish embryo
  • drosophila embryo, puppa
  • Arabidopsis seedling root
  • anything transparent up to 5mm

 

 


Thursday September 18, 2014

Director:
Dr. Eva Johannes
(919) 515 3525
johannes@ncsu.edu

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Arabidopsis seedling GFP
   
 
Page last updated on April 7, 2014 by EJ