Stephanie King , BS , MS
slholsen@ncsu.edu

Education

• NORTH CAROLINA STATE UNIVERSITY                                                  Raleigh, NC  
• Master of Science, Molecular and Structural Biochemistry                        May 2006         
• Taught biochemistry and developed further lab techniques including: colony growth, mutagenesis, recombinant plasmid development, cloning, protein expression, PCR, FPLC, protein isolation and purification, transformation/transfection, protein crystallography, DNA isolation and mutation, SDS-PAGE, Western Blot, X-Ray Crystallography, and UV Spectroscopy.
• APPALACHIAN STATE UNIVERSITY                                                        Boone, NC           
• Bachelors of Science, Biology (Minor in Chemistry)                                   May 2002          
• Lab techniques studied: thin layer chromatography, small organic molecule synthesis, basic bacterial analysis, negative staining, gram staining, spore staining, acid fast and simple staining, Ames test, Kirby-Bauer Method, gel electrophoresis, Southern Blot, and automated sequencing using the Sanger method.
• Computer techniques: Vector NTI Suite Software, introduction to BLAST, Homology, and Gen Bank searches. 

Professional Experience

Research/Lab Technician                                                                                 Winston-Salem, NC
 2001-2003  Wake Forest University Baptist Medical Center                                                  
Responsibilities included data collection, organization, and background research for orthopedic oncology.  Determined and insured research/experiments followed internal review board protocol.    

Research/Lab Technician                                                                                        Greensboro,NC
 2000-2001 Vita Olympic                                                                                

Performed small scale reactions to insure success on large scale and also tested physical properties of the finished product to examine quality and consistency.

Awards

• President and member of the Equestrian Club, Appalachian State University 1998-2001
• Member of the biology honor society Tri Beta, Appalachian State University 2000-2002
• Member of honor societies: Gamma Beta Phi and Alpha Chi, Appalachian State University, 2000-2002
• Graduated Cum Laude with 3.5 G.P.A.

Publications

Metastatic Disease of the Femur: Surgical Treatment.  Clin. Ortho.  William G. Ward, MD; Stephanie Holsenbeck, BA; Frederick J. Dorey, PhD; Jeff Spang, MD; David Howe, MD.  Issue 415. October 2003. H-Ras and its Oncogenic Mutants Ras G12V and Ras Q61L, Thesis.  North Carolina State University, 2006.

NC State College of Veterinary Medicine
Comparative Pain Research Laboratory
4700 Hillsborough Street
Raleigh, NC 27606