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The 17th Annual

NC State University
Undergraduate Research Symposium

 

Biological Sciences:

Ecology, Environmental, Conservation, Botanical

Abstracts

 


Abstracts are listed in alphabetical order by the last name of the corresponding author.

 

 

 


 

 

 

  • Biological Sciences abstracts

Applied Sciences (Crop, Poultry, Animal, and Horticultural Sciences)

Ecology, Environmental, Conservation, Botanical
Molecular, Biochemical, Genetics, Cell Biology
Zoology, Physiology, Behavior, Neurobiology

 

 

 

 


 

Student Author(s): 

Abbott, Jessica M.

Department(s):

Zoology

Research Mentor(s)

Nick M. Haddad/Zoology

Title of Presentation:

Host Plant Preference in Neonympha areolata

 

The Georgia Satyr (Neonympha areolata) is a butterfly found throughout the southeastern US that is closely related to the endangered Saint Francis Satyr ( Neonympha mitchellii francisci ). While there have been previous studies of adult N. areolata and N. m. francisci , the larvae of neither species has been observed in the wild and, as a result, little is known about their larval development and feeding behaviors. Larval host plant specificity has been well documented in many lepidopteran species. We investigated host plant preference in N. areolata larvae by placing eggs from N. areolata females found at Fort Bragg Military Installation, NC in pots containing the choice of two possible host plant species. Four sedge/grass species (C. mitchelliana, C. lurida , C. atlantica , and C. brevipilis ) were tested as potential host plants. There were two replicate pots for each of the six possible combinations of plant species. We recorded the proportion of larvae feeding on each plant within each pot every three to four days until larvae pupated. We used maximum likelihood to estimate the relative attractiveness of each plant species to the larvae. We used similar methods to estimate the survivorship of larvae on each plant species. We found that C. mitchelliana appeared to have the highest attractiveness. Interestingly, C. micthelliana also had the lowest estimated survivorship. Survivorship estimates were consistent with data collected in a previous study of N. areolata larvae survival when reared on a single host plant. Our study of N. areolata larval ecology serves as a pilot study for similar efforts with N. m. francisci larvae. Observing the feeding behaviors and survival of larvae in captivity gives insight into larval habitat requirements in the wild, which can be directly applied to conservation efforts, such as habitat restoration and augmentation. 

 


 

 

Student Author(s): 

Adams, Gina R.

Department(s):

Garner Magnet High School

Research Mentor(s)

Ann Cutter/General Education (Biology), Randolph Community College

Title of Presentation:

Amphibian Mutations

 

 

We researched further on amphibian mutations, because a mutation of any sort to a human or a different organism usually indicates something wrong in the environment. According to the journal articles the researcher read, there are 3 possible reasons for amphibian declines: an increase in UV- B radiation resulting from ozone layer depletion, chemical contamination: the effects of pesticides, acid precipitation, or the effects of fertilizers and herbicides, introduction of exotic competitors and predators. Previous research suggests the above mentioned, so the researcher attempted to induce mutations amongst the tadpoles by using: fertilizer, herbicides, UV- B radiation, Bayer aspirin pills. The question posed was what environmental factors are causing amphibian mutations. Based on the results, the researcher had to modify the question. There was one control tank, and the rest were experimental. A total of 10 tanks, each one with about 25 eggs, and each labeled by letter so as to identify which tank had which variable when recording observations. The variables added to each tank were representative of pollutants that might flow into the normal habitat of a developing frog. The response to all pollutants was the same: all the frogs died. The only thing that differed was the rate at which each tadpole died due to the unstable temperatures, too much of or lack of natural light, and the different variables added to each tank. I had ten trials total. I've found that the experiment left me with more questions than answers, but that these questions would be helpful in future research.

 


 


 

 

Student Author(s): 

Alderman, Joe D.

James, Jackie 

Gregory, Drew

Watkins, Arthur

Cooper, Jeremy

Department(s):

College of Natural Resources

Research Mentor(s)

Chris DePerno/Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Caitlin Burke/Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Title of Presentation:

Carolina Heelsplitter in Peril

 

 

In the United States, there are ~300 species of freshwater mussels (family Unionidea), nearly 1/3 of the world’s population.  Of the 300 species, ~70% are threatened or endangered.  One of the most imperiled species is the Carolina Heelsplitter (Lasmigona decorata), a federally endangered mussel located in North and South Carolina.  Populations of this rare mussel have suffered extreme habitat loss from sedimentation, pollution, and riparian buffer loss.  Competition with invasive species has also impacted the recovery of the Carolina Heelsplitter.  Currently, only six populations of the Carolina Heelsplitter are known to exist and are limited to the headwaters of small streams and creeks.  In North Carolina, the range of the Carolina Heelsplitter is limited to two creeks, Waxhaw Creek and Goose Creek.  We created a public opinion poll to determine the importance of the Heelsplitter to the general public. Surveys were conducted at convenience stores in two North Carolina Counties: Johnston County, ~1.5 hour driving distance from Goose Creek, and Union County, ~10 miles from Goose Creek.   The results indicate a similar knowledge of the Carolina Heelsplitter’s existence, a willingness to change practices to help the endangered species, an understanding of the current drought conditions, and support for public education related to endangered species.  Of the populations polled, ~10% have heard of the mussel, a number that should be increased through public education.   Education is of utmost importance in the conservation of the Carolina Heelsplitter.  The human population in the surrounding areas of Goose and Waxhaw creek is large enough to have a significant impact on the watershed.  Most people have a tendency to not change household and land management practices if they don’t understand the potential impacts those practices may have on flora and fauna.  The people we polled showed willingness to change, but not the knowledge.   

 

 


 

 

Student Author(s): 

Bhandari, Rohit

Department(s):

Plant Biology

Research Mentor(s)

William Hoffmann/Plant Biology

Renee Marchin/Plant Biology

Wade Wall/Plant Biology

Matthew Hohmann/ERDC-CERL

Title of Presentation:

Comparative Ecology of Rare and Common Amorpha Species

 

 

Understanding the rarity of plants caused by different biological factors can help us preserve plants that are on the verge of extinction.  We used the genus Amorpha as a model to study the rarity of plants.  We did a comparative study with the common species A. canescens, A. fruticosa, A. herbacea, A. nana, and the rare A. georgiana var. georgiana, A. georgiana var. confusa and A. schwerinii.  We hypothesized that the rarity with the less common species is caused by sensitivity to shade conditions.  We compared the growth patterns of the seven species in response to differences in light levels.  Plants were grown under 90% shade or full sun light.  The shade treatment was used to mimic tree encroachment which may be an important cause of rarity in fire suppressed habitats.  The plants were watered with nutrient solution every other day and the carts were rotated every other day.  We measured dry seedling mass and leaf area shortly after germination, as well as at 50 and 100 days after germination.  We used this data to perform a classical growth analysis to compare the rare species to the more common species.  We calculated relative growth rate, leaf area ratio, leaf mass ratio, specific leaf area, and net assimilation rate.  With these results we hope to understand the effects of shading and if morphological traits influence rarity.  This study should provide usefully information for land managers who work to protect rare Amorpha species from extinction. 


 


 

 

Student Author(s): 

Burkey, Mary T.

Department(s):

Forestry and Environmental Resources

Research Mentor(s)

Terrie Litzenberger/Forestry and Environmental

Resources

Title of Presentation:

Carbon Dioxide as an Indicator of Indoor Air Quality in Wake County’s Classrooms

 

 

Carbon dioxide itself is not a health threat at levels normally found indoors. Being a by-product of respiration, carbon dioxide is an indicator of low ventilation rates and related indoor pollutants in classrooms.  In this study, six classrooms in two high schools and a middle school in Wake County were measured for indoor air quality parameters. Carbon dioxide, temperature, and humidity were selected as indicators of indoor air comfort and proper ventilation.  Carbon monoxide was measured because it is used as an indicator of combustion contaminants in indoor air.  Teachers in each of the classrooms recorded the number of students in the room at intervals during the day and included comments on the indoor air comfort.  The results indicated that 83 percent of the classrooms exceeded the ASHRAE recommended level of 1000 ppm carbon dioxide in before 2007 at least once during the three day period.  33 percent exceeded 1500 ppm carbon dioxide and 17 percent surpassed 2000 ppm. When the levels of carbon dioxide were compared with the occupancy of the room over time, there was an increasing trend between the two. The carbon dioxide levels decreased to expected levels when the occupancy of the room was zero. The temperatures ranged from 29.2 to 15.8 degrees Celsius. The humidity ranged from 60.1 to 13.5 %RH. The teachers recorded complaints of temperature fluctuations, headaches, and feeling that the classroom air was either “dry” or “stuffy.”   Older, closed classrooms with a high occupancy had increased levels of carbon dioxide, thus potentially poor air quality and inadequate ventilation. Indoor air quality relates to student concentration in the classroom. With the escalating population of Wake County and the aging and physical degradation of the school buildings, the indoor air quality of the schools have the potential fall to unhealthier levels if not properly maintained. 

 


 

 

Student Author(s): 

Clark, Bethany

MacLennan, Donna

Register, Brent

Byrd, Adam

Griffin, Jessie

Department(s):

Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Research Mentor(s)

Chris DePerno/Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Caitlin Burke/Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Title of Presentation:

Promoting Native Plant Species for Landscaping

 

Invasion by non-native plant species is considered the second most dangerous threat to biodiversity.  In the United States, non-native plants invade approximately 700,000 hectares of wildlife habitat each year by out-competing native plants (i.e., originating naturally and evolving with wildlife) through superior reproduction, quick growth, and alleopathic qualities.  Non-native plants did not originate in a particular area nor evolve with the wildlife.  Non-native plants may be introduced accidentally through foreign imports or intentionally for erosion control, landscaping, or as a wildlife food source.  Additionally, some non-natives can alter ecosystems, transport diseases, negatively impact wildlife habitats, food sources, land management, microbial soil communities, and economies.  Unfortunately the general public is often unaware of the differences between native and non-native plants.  Our study focused on the ecological problems associated with non-native plants. We surveyed 150 landowners who utilize plant nurseries for landscaping.  The surveys were conducted at three nurseries known to stock native plants and three large retail stores with garden centers.  Survey results revealed 52% of the respondents were not looking for a specific plant, 95% were willing to consider a native plant for landscaping, and 89% were interested in learning more about native plants.  Thirty-five percent of landowners stated their plant choice was influenced by aesthetics, 33% by conservation, and 31% stated both were equally important.   The results of our survey indicate an opportunity to educate landowners about the benefits of native plants and the negative impacts of non-native plants.  Promoting landowner use of native plants for landscaping can help support native wildlife diversity and healthy ecosystems. 


 


 

 

Student Author(s): 

Frock, Catherine F.

Department(s):

Zoology

Research Mentor(s)

Nick M. Haddad/Zoology

Nicole Y. Thurgate/Zoology

Laura S. Vogel/Zoology

Title of Presentation:

How Do Wildfires Affect the Terrestrial Movement Behavior of Amphibians?

 

The use of fire as a habitat management technique is common in many forest ecosystems. At the Fort Bragg military installation, ephemeral ponds are located within longleaf pine forests which are regularly burned by managers. Eastern tiger salamanders (Ambystoma tigrinum tigrinum), which are threatened in North Carolina, and other amphibian species such as ornate chorus frogs (Pseuadcris ornata) use these ponds for breeding and live within the surrounding woodlands as adults. During breeding seasons, they must travel through woodlands to reach the ponds. Although aquatic breeding habitats are often the focus of management efforts for amphibians, the quality of terrestrial habitats is essential to consider in any conservation plan. Wildfires can serve as a model for understanding how fire may change the movement behavior of amphibians in woodlands. My research objective was to determine how wildfires affected amphibian movement behavior by tracking animals exiting three breeding ponds surrounded by forested habitats, one of which was burned immediately prior to breeding migrations and two of which had not been recently burned. To trace individuals’ movements, fluorescent dye powder was applied to the ventral surface of animals and deposited onto landscape features as animals moved. The resulting trails were followed using ultraviolet flashlights and marked at regular intervals until powder was no longer detectable. Ornate chorus frogs traveled significantly shorter distances in non-burned habitats, meaning that either: (1) they moved differently in the burned habitat or (2) my ability to detect trails was greater in the burned habitat. A future study will experimentally assess the reason for this variation and will provide important information on both the usefulness of powder-tracking as a non-invasive way to study amphibian movement and on the response of amphibians to landscape changes caused by fire.

 

 


 

 

Student Author(s): 

Fairey, Donta' J

Department(s):

Forestry and Environmental Resources, Environmental Technology Program

Research Mentor(s)

Stacy A. C. Nelson/Forestry and Environmental Resources

Title of Presentation:

Urban Land Cover Classification from High Resolution Imagery

 

 

Accurate interpretation of land use is a key factor in managing our natural resources. Land cover change is inevitable and affects a number of different ecosystem values including biodiversity, hydrology and biochemical cycles on various scales. Urbanization is a common factor contributing to increased land use change. Communities located within close proximity of a watershed as an example, could have adverse affects resulting from urbanization. Urbanization often results in land being converted from a condition that is permeable by water to one that is impermeable, or impervious, to water. An increase in impervious surface, in turn, adversely affects terrestrial and aquatic environments. Scenarios include increased run off from impervious surfaces and increased sediments in local drinking water supply. Several efforts have been implemented to assist land use management with these issues. An effective means of doing so has been through the use of a Geographic Information System (GIS), and high res imagery via aerial photography or satellite. GIS is a software based system that allows user to store, record, and analyze features and patterns on the earth’s surface. In using this technology decision makers can pro-actively address land use change issues in a timely manner. The objective of this project is to explore classifications techniques using satellite imagery to assess the impact of increased urbanization on water quality. 

 

 

 

 


 

 

Student Author(s): 

Fiza, Shaharyar

Department(s):

Biological Sciences

Research Mentor(s)

Larry F. Grand/Plant Pathology

Marc A. Cubeta/Plant Pathology

Title of Presentation:

DNA-based Identification of Wood Decay Fungi

 

  

Wood decay fungi play an important ecological role as decomposers of wood. These fungi cause deterioration of wood in both living and dead trees. They degrade cellulose and lignin in wood and convert them into usable forms of nutrients for their growth and development, and for use by plants and other microorganisms. The objective of this study was to develop a DNA sequence-based method to identify six species of wood decay fungi, Fomes fomentarius, Fomes fasciatus, Piptoporus betulinus, Schizopora apacheriensis, Schizopora flavipora, and Schizopora paradoxa. Experiments were conducted with pure cultures obtained from the USDA Forest Products Laboratory culture repository in Madison, Wisconsin or by isolating the fungus from fruiting bodies sampled from trees in North Carolina and Pennsylvania. Genomic DNA was extracted from each sample and the internal transcribed spacer region of nuclear-encoded ribosomal RNA gene (rDNA) was amplified with the polymerase chain reaction (PCR). Amplified PCR products were sequenced and compared with published DNA sequences in National Center for Biotechnology Information (NCBI) database GenBank. Sequences from each of the six fungi were at least 95% similar in sequence identity to representative sequences of these fungi in GenBank. Additional experiments were conducted to develop a method to extract, amplify and sequence DNA directly from the fruiting bodies of F. fomentarius and F. fasciatus from fresh field collected and herbarium samples. Sequences from fresh and herbarium samples had 97% sequence identity to cultures isolated from fruiting bodies and sequences in GenBank. The high level of nucleotide sequence similarity of F. fomentarius and F. fasciatus to known sequences confirmed their identity. The method developed in this study for field collected and herbarium samples will be helpful for identifying other wood decay fungi to better understand their ecology.

 


 

 


 

 

Student Author(s): 

Godfrey, Charles R.

Department(s):

Biological Sciences

Research Mentor(s)

Thomas Wentworth/Plant Biology

Wade A. Wall/Plant Biology

Matthew Hohmann/Ecological Processes Branch, ERDC-CERL, Champaign, Illinois

Title of Presentation:

The Effect of Fire Regime on the Fecundity of Pyxidanthera brevifolia

  

 

Pyxidanthera brevifolia (Sandhills pyxie-moss) is a small sub-shrub endemic to longleaf pine ecosystems in the southeastern United States, found only in four counties in North Carolina and two in South Carolina. Very little is known about its growth cycle and fecundity. The longleaf pine ecosystem, which at one time thrived throughout the southeastern United States, depends on the cycle of natural wildfire for its maintenance. At the Fort Bragg military installation in the sandhills of Cumberland and Hoke Counties, North Carolina, the Federal Government supports the maintenance of such an ecosystem by managing a regular fire regime, burning each sector of the ecosystem every three years. Fort Bragg is currently changing its three-year burn cycle to a two-year cycle, and it is uncertain of the effect this could have on P. brevifolia’s fecundity. We measured the percentage of flowering individuals at 23 burn sites representing the three levels within the current regime (3, 2, and 1 year(s) since last fire). We also measured the ratio of leaf litter to surface area for each burn site to investigate the relationship litter has to the burn cycle and fecundity.  The sites burned one year ago had significantly lower flowering percentages than those burned two and three years ago. This raises the concern that a two-year burn regime might significantly reduce the fecundity of P. brevifolia in this ecosystem. Further research should be conducted, specifically examining the role of leaf litter and other potential effects on flowering, in order to construct a more accurate growth model.

 


 

 


 

 

Student Author(s): 

Gomes, Kerrel

Department(s):

Environmental

Research Mentor(s)

Chris DePerno, Forestry and Environmental Resources, Fisheries and Wildlife Science Program

Title of Presentation:

How Does Dissolved Oxygen in Local Streams Affect Invertebrates and Water Quality?

 

 

I studied the connections between three specific invertebrates and water quality. I focused on the Caddis Fly, Stone fly and Mayfly and the types of streams they live.  I conducted my sampling at a stream on the NCSU campus.  I recorded oxygen levels, conductivity and temperature of the water. This analysis was essential to determining if a majority of macro invertebrates would find these streams suitable for living. The invertebrates live in water with high oxygen levels. Using a dissolved oxygen and conductivity meter we measured rocky flowing areas and pooling areas of the stream. I conclude that oxygen levels were best in the flowing areas of the stream.   Also, the water quality and temperature was best in this area because cold water holds more oxygen than warm water.

 


 

 

Student Author(s): 

Grimes, Shavon R.

Department(s):

Pulp and Paper Science

Research Mentor(s)

Lucian Lucia/Pulp and Paper Science

Title of Presentation:

A Preliminary Investigation of Pocosin-Derived Sweetbay Essential Oils against E. coli

 

 

The purpose of my project is explore antibacterial chemicals in plant oils. To determine whether or not the chosen plants have antibacterial oils, we tested a composite sample of extracted oils against E.coli. Not only did we test the native plant oils, we also tested the constituent major pure oils of the composite native sample. We extracted plant oils using hexane and water, then applied them to a 1 centimeter disk of filter paper, put each disk on agar swabbed with E.coli bacteria and followed the behavior of the bacterial lawn. We then compared the antibacterial activity of the native plant oils growth to the pure oils for the purposes of ranking the activity of the main components (pure oils) to the native mixture (various proportions of pure oils). Changes in the bacterial growth give us insight into the antibacterial properties of the oils in both the plant and the main (pure) oil constituents.

 

 


 

Student Author(s): 

Hanson, Christopher A.

Department(s):

Civil Engineering

Water Resources

Environmental Engineering

Research Mentor(s)

Detlef R.U. Knappe/Civil Engineering; Water Resources; Environmental Engineering

Morton A. Barlaz/Water Resources; Environmental Engineering

Title of Presentation:

Anaerobic Toxicity Assay of Malathion and Malathion

Hydrolysis Products Co-disposed with Simulated Building Debris

 

 

The behavior of organic contaminants in landfills is governed by several processes including biodegradation, sorption, volatilization, leaching and abiotic hydrolysis. The overall objective of this research program is to develop and validate a predictive model of organic contaminant fate and transport in landfills. One aspect of this model is measurement of biodegradation and toxicity. Model validation will be conducted with malathion as a model compound because it undergoes both abiotic and biological degradation processes. The specific objectives of this research were to determine if malathion and malathion hydrolysis products will inhibit decomposition of biopolymers present in simulated building debris (SBD) and to identify the concentration of malathion that will be toxic to an anaerobic microbial consortium enriched from decomposed municipal solid waste (MSW). SBD was used to simulate a scenario in which the contents of a building are contaminated and must be disposed in a landfill. An anaerobic toxicity assay (ATA) was conducted in reactors that contained a defined medium, ground SBD, an inoculum, and malathion or ethyl hydrogen fumarate (EHF)/diethyl hydrogen fumarate (DEF) and malathion monocarboxylic acid (MCA). The ground SBD contained ceiling tiles (14.7%), medium density fiberboard (38.4%), carboards and folders (4.8%), and white paper (39.2%). Plastics were excluded to eliminate sorption. The reactors were incubated at pH 7.1, 37°C for 60 days. Gas production was measured based on the overpressure from the headspace in the serum bottle. The average methane yield was 97.1 ± 9.6 mL/g SBD at STP, which is 41% of the theoretical methane potential (235.8 mL at STP/g) based on the cellulose and hemi-cellulose content of the SBD biopolymers. At the 95% confidence level, malathion and malathion hydrolysis products (EHF, DEF, MCA) at 1 to 20 mg/L did not affect activity of the anaerobic consortium enriched from decomposed MSW and did not affect SBD biopolymer decomposition.

 


 


 

Student Author(s): 

Izzi, Vincent S.

Department(s):

Environmental and Molecular Toxicology

Research Mentor(s)

Gerald LeBlanc/Environmental and Molecular Toxicology

Title of Presentation:

Modeling Toxicodynamic Effects of Malathion and 4-Chlorophenol

 

  

Environmental toxicology is the study of the fate and effects of chemicals in and on the environment. This experiment utilized two environmental chemicals, malathion and 4-chlorophenol, and tested how they reacted together. These two chemicals were exposed to the invertebrate Daphnia magna in an aquatic environment. There are two different toxic effects that the two chemicals can elicit together: an additive effect and a multiplicative or synergistic effect. The synergistic effect can be divided into toxicokinetic and toxicodynamic. Additive and toxicokinetic effects have both been computer-modeled. It was the point of this experiment to model a toxicodynamic effect. It was predicted that malathion and 4-chlorophenol would produce a synergistic toxicodynamic effect. The results found in the preliminary data supported the hypothesis that malathion and 4-chlorophenol did produce a synergistic effect. The experimental data seemed inconclusive as to whether or not a toxicodynamic effect was occurring between the two toxicants. Either more replications need to be produced for statistical assurance or the mechanism of toxicity needs to be reconsidered. Because the data did not support the hypothesis of a toxicodynamic effect between malathion and 4-chlorophenol, the computer-modeling step was not taken.

 


 


 

Student Author(s): 

Lancaster, Kendall M.

Department(s):

Water Quality

Research Mentor(s)

Zach Q. Chen/NC Dept. of Water Quality

Shaefny Grays/College of Natural Resources

Title of Presentation:

Quality Trends in Falls Lake, Raleigh, North Carolina during March 2005 to September 2007

 

 

Analysis of nearly 7,000 physical and chemical water quality data, which were collected by the North Carolina Division of Water Quality from its ambient monitoring stations of NEU018E and NEU020D during March 15, 2005 to September 19, 2007, show that the general trend of water quality in Falls Lake, the drinking water reservoir of the City of Raleigh, is declining during the period of time. Physical data reveal that during the 2.5-year period of time, the trend of the *overall* water temperature in Falls Lake rose at an alarming rate of 0.4~*0.92* degree-C per year; the *surface* water temperature trend rose at a *much faster* rate of approximately 1.2~1.33 degree-C per year. The trend of the dissolved oxygen level in the lake declined at a rate of approximately 0.17~0.3 mg/L per year; the trend of pH declined at a rate of 0.06~0.13 per year, and the trend of the surface water conductivity declined at a rate of 7.5~12 umhos/cm per year. The trend of water visibility remained basically the same. Biological data show that the nutrients -- Total Phosphorus (TP) and Total Nitrogen (TN) in the lake water -- fluctuated over time, due to seasonal and weather changes. Nevertheless, the general trends of both TP and TN remained basically the same, or even slightly declined.  The trend of the total solids concentration in the lake water appeared slightly declining at a rate of approximately 2.4~3.2 mg/L per year while the water turbidity trend at the both stations rose at a rate of 0.8~0.9 NTU per year. Chlorophyll-a trend demonstrated a strong local variation, characterized by a 0.82 ug/L/year rise at station NEU018E and a 2.52 ug/L/year declining at station NEU020D. 

 


 

Student Author(s): 

Levine, Brian M.

Department(s):

Environmental Technologies

Research Mentor(s)

Elizabeth Guthrie Nichols/Environmental Technologies

Title of Presentation:

Does Plant Organic Matter Change Equilibrium Partitioning (Log Koc) of Select Polycyclic Aromatic Hydrocarbons (PAHs) Between Water and Petroleum-contaminated Sediments?

 

Plant organic matter may stimulate soil/sediment microorganisms to degrade polycyclic aromatic hydrocarbon (PAH) contamination in the environment. The goal of this project is to measure the organic carbon normalized equilibrium partitioning coefficients (Log Koc) of select PAHs (phenanthrene and pyrene) between sediment and water using two compartment, aqueous batch systems. The goal of this project is determine if Plant organic matter alters PAH binding to sediment organic matter (SOM). These measurements will help determine if the influx of plant organic matter alters Log Koc values for phenanthrene and pyrene. Five grams of air-dried contaminated sediments were added to 250 mL amber jars and filled completely with 0.01M CaCl2 solution that contained NaN3 as a biocide. Single-point organic carbon-normalized distribution coefficients (Koc) from aqueous desorbed PAH concentrations (phenanthrene and pyrene) (Cw) were measured after 1 month of equilibration. The aqueous portion was decanted and allowed to sit for 24 hours. An aliquot of the decanted aqueous phase was removed by pipette and extracted with dichloromethane (DCM). Total extracted PAH from the sediment fraction (Cs) was determined by solvent extracting sediment fractions after aqueous desorption. DCM extracts were dried and prepared for GC/MS analysis. Koc values were normalized to the organic carbon content in the sediment fraction in order to compute an organic carbon-referenced partition coefficient Koc (L/g OC).

 


 

Student Author(s): 

Molin, Jasmine M.

Department(s):

 

Research Mentor(s)

Carly Carroll/Atmospheric Protection Branch

Title of Presentation:

Invasive Species in North Carolina

 

 

I researched invasive species in North Carolina. I found that invasive species affect our native species everyday. Yet the timing we did this experiment was during the winter and we were/are in a drought which effect the different species. For our experiment I took off a certain space, or area, and took samples of different species. This included taking pictures of the different species. Later I identified the species that were found and checked if they were native or invasive.  Since the timing of this was a hard time to find invasive plants and animals, there was extra research done to find out where most indigenous species are found in North Carolina and the effects as well as what they are trying to do to prevent certain problems caused by these species.

 

 


 

Student Author(s): 

Raybuck, Amy L.

McAllister, John M.

Brown, Jason S.

Bustard, Greg T.

Beasley, Nathan W.

Department(s):

Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Research Mentor(s)

Chris DePerno/Forestry and Environmetal Resources, Fisheries and Wildlife Sciences Program

Caitlin Burke/Forestry and Environmetal Resources, Fisheries and Wildlife Sciences Program

Title of Presentation:

Compact Fluorescent Light Bulbs and the Hidden Cost of Mercury Contamination

 

A principal threat to the environment is mercury pollution through anthropogenic sources. When mercury is released into the environment, it becomes available for uptake by organisms, including humans, leading to bioaccumulation in the food web. Increased mercury levels could lead to heavy metal poisoning or developmental problems in fetuses and children. Coal-fired power plants are the primary source of mercury pollution and reducing emissions would have positive implications for the environment and human health. To reduce emissions, environmentalists are advocating for the use of compact fluorescent light bulbs (CFLs) which use less energy and last longer than incandescent light bulbs. Unfortunately, mercury is one of the main components of CFLs. When the bulbs are disposed in traditional landfills, mercury could leach into the environment and contaminate watersheds. Consequently, CFLs require proper disposal at hazardous collection sites. The CFLs that consumers purchase lack warnings and disposal instructions, and as a result, consumers are ignorant of mercury pollution and disposal procedures. To determine the extent of people’s knowledge about CFLs and proper disposal, we administered a survey to 121 students in the College of Natural Resources at North Carolina State University. Our results indicate a lack of knowledge about correct disposal of CFLs. Of respondents who used CFLs, <2% knew how to properly dispose of the bulbs. We believe that priority should be given to education and awareness of CFLs, with emphasis on their benefits, threats and proper disposal. This responsibility should lie with all companies and groups advocating for their use, especially manufacturers and distributors of CFLs. 

 

 


 

Student Author(s): 

Rindos, Irena

Department(s):

College of Natural Resources

Research Mentor(s)

George R. Hess/Natural Resources

Title of Presentation:

Changes in Raleigh's Urban Forest Canopy Following Development

 

Urban forests provide important ecological services to cities, but development often reduces the health and size of urban forests. The extent to which an area of urban forest declines and recovers after development thus has important implications. I used aerial   photographs of Raleigh to estimate tree canopy and track changes in canopy cover before and after development.  The date an area was annexed was used to   estimate when it was developed. Areas of Raleigh annexed between 1980 and 1989   were chosen for analysis based on the availability of aerial photographs before   and after development. I used 1200 random sampling points to estimate tree canopy for the   years available.

 


 

Student Author(s): 

Shearin, Margaret E.

Department(s):

Plant Biology

Research Mentor(s)

Thomas R. Wentworth/Plant Biology

Kristen M. Kostelnik/Plant Biology

Janet B. Gray, Dept. of the Army, Natural Resources Division, Fort Bragg, NC

Title of Presentation:

Analysis of the Spatial Distribution Patterns of Astragalus michauxii, andhills milkvetch

 

 

The number of threatened and endangered plants and animals is dramatically increasing.  Conservationists often know little about these species’ habitats and life histories, information essential to effective protection plans.  A first step to discovering a species’ habitat and potential limiting factors is characterization of spatial distribution.  Whether a species exhibits a clumped, uniform, or random distribution leads to different questions in continuing studies.  Clumping implies constraints by physical environment or dispersal, uniformity implies strong competition between individuals for a limiting resource, and randomness implies average dispersal with weak or no limiting factors.  The sandhills milkvetch (Astragalus michauxii), a rare perennial herbaceous plant in the family Fabaceae, occurs only in longleaf pine sandhills ecosystems.  Little is known about its spatial distribution or limiting factors within preferred habitat.  Observers first assumed A. michauxii grew uniformly.  We chose quantitative methods to ascertain its spatial distribution in fire-maintained longleaf pine savannas in Fort Bragg Military Reservation (North Carolina).  We determined nearest-neighbor distances among individuals of A. michauxii in three subpopulations that were part of a more comprehensive population-level survey.  Analysis of the nearest-neighbor data using Clark and Evans’ method (1954) revealed a statistically significant clumped pattern among individuals within each subpopulation.  On a larger scale, we also found a clumped pattern among the subpopulations of A. michauxii within the study area (Fort Bragg).  To determine if spatial proximity among individuals within subpopulations might have consequences for plant size and reproductive effort (stem and seed capsule number) or herbivore consumption (capsule predation number and percentage, defoliation extent), we regressed these attributes against nearest-neighbor distance, but found no statistically significant relationships.  Our preliminary conclusion: proximity to other conspecific individuals is unrelated to plant growth or susceptibility to herbivory.  Further investigations can now focus on why individuals and populations are clumped, based on limiting factors and dispersal methods. 

 

 

 


 

Student Author(s): 

Smith, Brandon C.

Department(s):

Agricultural Life Science

Research Mentor(s)

Julia Clarke/Marine, Earth, and Atmospheric Sciences

Title of Presentation:

Reevaluating Morphological Decay Stages of Green River Birds

 

 

Taphonomy is the study of the processes an organism undergoes after its death, burial, and subsequent discovery as a fossil.  This includes decay, transport, and other chemical, biological, or physical influences that affect the remains of an organism and remains the largest source of information prior to that organism’s deposition into the fossil record.  Understanding the impacts of taphonomy is crucial before interpretations of the depositional environment or the organism’s life-history may be made.  An analysis of an actualistic study examining the morphological decay of modern birds as a correlate to fossil birds reveals several incongruities that must be resolved.  To solve these problems a new method of scoring was constructed and tested through rigorous application.  Fossil birds recovered from the Green River formation were chosen to test this experimental method for their high degree of preservation and relative importance in the paleontological record.  In this method, over eighty fossils were carefully examined.  Bones that preserved and the specific points of articulation between them were identified and recorded.  Other points of interest such as fossil locality, the facies they were removed from, feather preservation, bone breakage, presence of tracheal rings and gut contents were noted as well.  In building and testing this framework, I have not only constructed a new method of scoring morphological decay in fossil birds but I have also discovered several trends among those of the Green River formation.  Such trends include the early loss of vertebral articulations, the preservational bias for bones of the wing and pectoral girdle, and greater feather preservation in mid-lake deposits. 


 

 


 

Student Author(s): 

Terrell, William G.

Department(s):

Environmentors

Research Mentor(s)

Sean Higgins/NC State Parks

Title of Presentation:

Macroinvertebrates and Water Quality: Urban Creek vs. Protected Park Stream

 

We investigated the relationship between water quality in Potts Branch, a stream in William B Umstead State Park, and in Perry Creek in north Raleigh. The macroinvertebrates (invertebrates you can see with your naked eye) living the two habitats (Potts Branch and Perry Creek) were very important for our study because the type and number of macroinvertebrates can be used to help determine if the stream habitat is healthy. We also collected basic data including stream width, average depth, water temperature and pH. The macroinvertebrates varied from riffle beetles to freshwater worms. Our materials included a dip net, test tubes, containers, gloves, measuring tape, a thermometer and litmus paper. We investigated if the water quality at different stream sites was poor, fair, good or excellent based on the macroinvertebrates we collected and identified. We found Crane fly larvae, Stone fly nymphs, Mayfly nymphs, Caddisfly larvae, Riffle beetles, Freshwater worms and Black fly larvae. Many of the macroinvertebrates found were intolerant of pollution, so we determined that the stream was relatively healthy in both locations. After analyzing the data, we concluded that the stream sites from the protected area in William B Umstead State Park contained more macroinvertebrates that are intolerant of pollution and more macroinvertebrate diversity.

 

 


 

Student Author(s): 

Urzen, Jessica M.

Department(s):

Zoology, Nutrition and Plant Biology

Research Mentor(s)

DeYu Xie/Plant Biology

Title of Presentation:

Comparison of Antioxidant Levels in Frozen and Imported Fresh Blueberries

 

In winter months when local fresh produce is not readily available, distributors rely heavily on imported products from other states and countries.  In the case of blueberries, consumers generally have two choices: frozen and imported fresh blueberries.  It is the general assumption that imported fresh blueberries contain higher levels of antioxidants when compared to its frozen alternative. Anthocynins are water-soluble vacuolar flavonoid pigments found in blueberries that act as powerful antioxidants.  The objectives were to determine and compare anthocynin levels in frozen and imported fresh blueberries.  Anthocynins were extracted from eight frozen and eight imported fresh blueberry samples from four major distributors in Raleigh; anthocynin quantity and type was measured.  Frozen samples yielded slightly higher anthocyanin levels than imported fresh samples using a UV spectrometer.

 

 


 

Student Author(s): 

Valdez, Siliza M.

Department(s):

USGS North Carolina Water Science Center, North Carolina State University-Asheville Water Quality Institute

Research Mentor(s)

Michelle C. Moorman/USGS North Carolina Water Science Center, North Carolina State University-Asheville Water Quality Institute

Title of Presentation:

Mercury Levels, Fish Consumption, and Human Health

 

Mercury contamination is increasing in the environment due to the increased use of coal burning power plants in the last century. This has increased the deposition of methyl-mercury in rivers and estuaries and has become a direct concern for aquatic and human health. Methyl mercury levels in freshwater and saltwater fish have increased, especially in large carnivorous fish, which are then be consumed by humans. High levels of mercury can have toxic effects on the human nervous system and other dangerous side effects. The purpose of my project is to examine how individual fish consumption impacts mercury levels in humans. I will accomplish this by conducting a survey that evaluates individual fish consumption habits and then testing the level ofmercury in the individual’s hair. This project will contribute to our understanding of how changes in our environment can impact human health.

 

 


 

Student Author(s): 

Williams, Kyanda S.

Department(s):

Wood and Paper Science

Research Mentor(s)

Carrie Hanayik/Wood and Paper Science

Title of Presentation:

Green Building

 

In my presentation I will be discussing the subject of Green Building. Topics will include information on green building, products that help, companies that support green building, etc.

 

 


 

 

Student Author(s): 

Williams, Abbey L.

Department(s):

Environmentors

Research Mentor(s)

Megan H. Mehaffey/U.S. EPA, Division of Environmental Sciences

Title of Presentation:

Land Use near Streams Can Have an Influence on Stream Condition

 

Garner, North Carolina has undergone many changes over the past 20 years including almost doubling its population. In addition to increased growth, Garner more recently has been experiencing extreme drought conditions. I believe the local streams have been affected by the recent drought and urbanization, including the pollution it has contributed. My mentor and I went into the field to collect macroinvertebrates in order to check the streams around Garner. Our collection did not yield any species of macroinvertebrates at the sites we selected. We noticed that most of the streams were located near or downstream from populated areas and some even had active construction. We concluded that the combination of drought and disturbance from urban growth may be having a negative affect on the stream environment. We will continue to research the other streams around the area to better answer our questions.

 

 

 


 

 

Student Author(s): 

Williams, Cassandra N.

Nunez, Marilena B.

Coxen, Christopher L.

Owen, Matthew G.

Cochran, Matthew

Department(s):

Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Research Mentor(s)

Chris DePerno/Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Caitlin Burke/Forestry and Environmental Resources

Title of Presentation:

Feral Cats: Implications, Management Strategies, and Public Opinion

 

The effect of anthropogenic carbon emissions into the atmosphere is a growing concern among the public.  To offset their carbon emissions the voluntary offset market was developed, allowing individuals to purchase credits that support carbon offset projects.  Our research describes the different forms of carbon offset projects, their advantages, and common criticisms.  We analyzed company websites that sell carbon offsets, sent an email questionnaire to companies that bought carbon offsets, and surveyed the general public.  Over half (59%) of the 22 companies analyzed do not provide an explanation of carbon offsets in their website literature, and the comprehension level for the explanations was 4 years of higher education.  Twelve companies that bought carbon offsets responded to our email questionnaire with a referral to their website, but half of the websites failed to adequately answer our questions.  We determined that our sample population had never heard of a carbon offset (48%) or heard of them but did not know what they were (22%).  However, most stated they were more likely to buy a product advertised as “carbon-neutral” and were willing to pay 10% to 25% more for the “green” product.  We believe the growing concern about harmful carbon emissions and the lack of quality information provided to the public by offset companies has left the public vulnerable to marketing manipulation and buzz words like “green” or “carbon neutral.”  Dedicated interest by an informed public, sound science, and consolidated regulation are integral to a quality offset market.  


 

 

 


 

 

Student Author(s): 

Wilson, James M.

Degnan, Jesse M.

Dycus, Justin C.

Eamick, Bonnie E.

Wagner, John David 

Department(s):

Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Research Mentor(s)

Chris DePerno/Forestry and Environmental Resources, Fisheries and Wildlife Sciences Program

Caitlin Burke/Forestry and Environmental Resources

Title of Presentation:

Feral Cats: Implications, Management Strategies, and Public Opinion

 

Domestic cats were introduced to North America in the 1500’s.  Since then, their numbers have grown steadily, and today, it is estimated there are between 65 and 100 million feral and free roaming cats in the United States.  A feral cat is defined as any cat that is unconfined, born wild, reverted to a wild or semi-wild state after being domesticated, or free-roaming.  Feral cats are efficient hunters and have been known to kill large numbers of birds, reptiles, amphibians, and small mammals.  Feral cats can transmit feline leukemia, feline immunodeficiency virus, and rabies within their colonies and to wild species (e.g., bobcats and mountain lions).  Feral cats can be managed by lethal or non-lethal options.  Lethal removal methods include poisoning and trap/kill, whereas non-lethal techniques include immunocontraceptives and trap-neuter-release. To assess public opinion regarding feral cats, we conducted a convenience survey of North Carolinians. Our objective was to determine differences between opinions of feral cats, their environmental impacts, and the level of approval for each control method in suburban neighborhoods, city parks, wildlife refuges, and national forests.  Our results indicate varying opinions on feral cat management options, ranging from no control to lethal removal.  In management scenarios where feral cats might transmit disease or themselves have a lower standard of health, the majority of respondents were supportive of controlling the cats.  However in management scenarios where other wildlife species are at risk the majority of respondents were not supportive of control methods.  These results indicate the need for public education on the impacts feral cats have on other wildlife species.  We believe that educational literature available at animal shelters, veterinary offices, and pet supply stores will increase awareness of the negative environmental impacts of feral cats.  Public venues (museums, libraries, schools) can be used to engage non-pet owners.


 


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Last modified January 2008  by Sharon E. Hunt, WordHunting