Salmonella is a major food borne or major cause of food borne disease.  Causes 1.4 or 1.5 million cases of food borne disease a year in the United States.  And of that poultry and egg products are one of major sources of food borne disease.  So, in order to eliminate that as a source of disease amongst people in the United States SalmonellaÕs an excellent candidate to go after.  So, the idea is since chickens are reservoir for salmonella, or so they harbor the strains of salmonella that make humans sick.  The idea is we can give them a strain of salmonella thatÕs not pathogenic and allow the chicken immune system to respond to the salmonella so that hopefully the chickens will clear salmonella from their systems, therefore there wonÕt be any salmonella in chicken products or food products by the time they make it to the consumer and therefore eliminate the likelihood that people get food borne disease from poultry products.  Vaccines for poultry typically have to be in the neighborhood of around a penny per dose.  Some are a little bit more.  Some are actually quite less.  And so if we can deliver vaccines or use vaccines that deliver immunity to multiple disease agents at once, it becomes much more cost effective.  And so with the recent push for the FDA and in controlling salmonella in poultry sort of demanding or otherwise at least driving the industry towards adopting these vaccines, if we can use a salmonella vaccine that provides protection for the consumer and at the same time that same vaccine provide protection against poultry pathogens that actually protect the animals as well, weÕll hopefully get a two for one bang for your buck out of the product.  This is essentially going to allow us to ask a lot of questions about how this vaccine works its ethicacicity which other types of pathogen antigens we can encode in the salmonella to use as the virus vector or a vaccine vector and actually sort of explore its utility and better develop it as an epicatious vaccine.  As this project develops and we demonstrate how well it works and that it may actually work be able to be used for more than just salmonella, companies, vaccine companies especially on animal health side of things will be interested in licensing developing and potentially producing this.  So, itÕll mean jobs for those producing the vaccine, but itÕll also quite possibly lead to increased revenues on the poultry side as well.  Which table eggs and egg products are and poultry in general is a major source of economic income for the state of North Carolina.  We decide to switch to salmonella because of its significance in food safety.  And we had also a colleague here in the department that used to work on salmonella.  So, itÕs like a teamwork.  And we started doing micro arrays study to look at the whole genome expression under different conditions.  And one of the conditions we discovered it also reduce the expression of the gene that cause disease.  So that, we call that strain as accentuating.  So, it acts like salmonella but it doesnÕt make you sick.  So, thatÕs a good hallmark for a vaccine strain.  So, our idea is we have a (unintelligible) little gene that doesnÕt regulate only one, regulate a lot of genes that involves invariance or making the organism a bad organism.  So, by deleting that gene in all the gene acquired for variance will be down regulated or not expressed well and that strain will be attenuated.  And this can induce immunity.  Again itÕs other strains of salmonella thatÕs variant.  So, thatÕs where the idea really came about.  All different routes.  The routes we are exploring is (unintelligible).  So, it can be added to the water or sprayed directly in the young chicks.  So, and thatÕs what they do nowadays is sprayed on the chicks and the chicks start licking each other and they get vaccination.  So, I cannot actually due to the lack of funding from further government and so on IÕve been funding the work of my lab for my own market.  So, the chancellor fund will help so I donÕt mind adding some of my own money as well.  So, itÕs I mean I can start a company but I really this technology if successful can increase employment in North Carolina and the whole United States and even overseas because itÕs a problem is not only USA problem itÕs a national international problem you know.